QUESTION 1 Why is heat or a VERY long incubation required to stain Mycobacteria? mycotoxins present in the bacteria break down the stain; heating destroys the mycotoxins allowing the stain to enter the cells. waxy mycolic acids present in the bacteria break down the stain; heat prevents the stain from breaking down. waxy mycolic acids present in the bacterial cell walls prevent the stain from entering at room temperature; when heated, some stain is able to penetrate the cells. mycotoxins in the bacteria prevent the stain from entering the cells; heating inactivates the mycotoxins QUESTION 2 Micrococcus luteus was used as a negative control in an acid fast staining procedure - what does it mean to be a negative control? THERE ARE 2 CORRECT ANSWERS O Negative control bacteria are NOT expected to stain with acid fuschin. Negative control bacteria are expected to stain. Negative control bacteria are expected to stain only when heated. A heat-fixed smear of negative control bacteria is an important comparison that should be present on a slide along with a heat-fixed smear of a postitive control, and of the unknown bacteria. 9 O 0:0 0

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Pathogenicity

Infection and Transmission

The infections are generated by the pathogenic organisms present in the environment. They maintain the capacity to invade a host body and establish colonies. A disease caused by such infectious agents is called a communicable disease or transmissible disease. These diseases spread through diverse means including blood, food, water, air, or vectors.