Question 1 Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume (mL) Total Protein (mg) Activity Activity Total Specific Percent Fraction Recovery 1. Crude extract 3,800 22,800 2,460 0.108 100 2. Salt precipitate 3. Ion-exchange chromatography 4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.
Question 1 Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume (mL) Total Protein (mg) Activity Activity Total Specific Percent Fraction Recovery 1. Crude extract 3,800 22,800 2,460 0.108 100 2. Salt precipitate 3. Ion-exchange chromatography 4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Question 1
Example of a Protein Purification Scheme:
Purification of the Enzyme Xanthine Dehydrogenase from a Fungus
Specific
Activity Activity Recovery
Volume
Total
Total
Percent
Fraction
(mL)
Protein (mg)
1. Crude extract
2. Salt precipitate
3. Ion-exchange chromatography
4. Molecular-sieve chromatography
5. Immunoaffinity chromatography
3,800
22,800
2,460
0.108
100
165
2,800
1,190
0.425
48
65
100
720
7.2
29
40
14.5
23
1.8
275
152.108
11
Calculate the specific activity of step#4. Note that percent recovery=% Yield.
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