None Question 1: (6 marks)The following diagram shows the parental DNA for two replication forks that are approaching one another from the left and right.(Note: Each linear line represents a single strand of DNA.)(a) Annotate the diagram (inside each fork) so that it accurately shows the result of nucleotide polymerization through the actionof the replisome, prior to any activity by DNA Pol I and DNA ligase. Be sure to label the polarity of all generated nucleic acids.Indicate by colour (eg powerpoint line colour) which sections represent DNA or RNA. For the leading strand, you can assume thatreplication processes started at the ends furthest away from the fork (4 marks)(b) Add a drawing of DnaB enzyme to the diagram to show where it would bind and its direction of movement if ATP were added tothis protein-DNA complex. (2 marks)5'3"Fork 13'Fork 25'

Answered: Image /qna-images/answer/e6e56cbe-b038-4c4d-8717-a439f53e453c.jpg

Answered: Question 1: (6 marks) The following…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question 9. What basal transcription factors can only bind to the DNA within the pre-replication complex after tata binding protein (TBP) bends the DNA?
There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA replication. a) Please replicate the parental strands into two exact copies TC GATATCGG AGCTATAGCC b) place a centromere between the two replicated copies (or tell me where the centromere would be located),
Question 1: Look at the following normal and mutant DNA sequences. Normal Sequence (5'-3'): ATG AAC GTT ATC GCA Mutated Sequence (5'-3'): ATG AAT GTC ATC GCA a) What type of mutation has occurred (be specific)? b) Fill in the table for the normal and mutated sequences. Starting with the given 5'-3' sequence, input the complementary DNA, transcribed RNA from the 3'-5' DNA and translated polypeptide sequence for both. Hint: use the codon table! Normal sequence Mutant sequence DNA 5'-3' (given) DNA 3'-5' RNA Polypeptide (use 3 letter codes) c) Based on parts A and B above, what is the ultimate effect this mutation has had on the polypeptide? (1 sentence summary)
Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support…
Question 7 Referring to the plasmid below, if a recombinant plasmid were obtained by inserting DNA into the EcoRv site, and the protein corresponding to the recombinant gene were expressed, which of the following statements would be false? Aval Sall A The plasmid will be resistant to tetracycline. B) The protein is not likely to be biologically active without some further treatment. C) The plasmid will be resistant to ampicillin. (D) The plasmid will not be able to replicate autonomously. Pul- Poul- ampr III EcoRI EcoRV BamHI pBR322 (4363 bases) -Poudl lett
Question 4. Given the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' and the GC content is 60%, c) Which of the following will lead to DNA cutting more frequently? Choose all that apply. O Increasing the AT content to 60% O Using a seven base cutter with the sequence 5'-GGATCCC-3' O Using a five base cutter with the sequence 5'-GGACC-3' O Increasing the GC content to 70% O Decreasing the GC content to 50%
Question 8. You isolate the DNA from the bacterial cells and apply the Sanger dideoxy sequencing method. You then separate the products of the reactions by gel electrophoresis and obtain the following pattern. What is the sequence of the template and the DNA on the gel? ddATP ddTTP ddCTP ddGTP Template V [ Choose 3'-CTAGTCAAGG-5' 5'-CTAGTCAAGG-3' Sequence 3'-GATCAGTTCC-5' 5'-GATCAGTTCC-3' 5'-GGAACTGATC-3'
Question 12 Referring to the pBR322 plasmid below, if a recombinant plasmid were obtained inserting DNA into the BamHI site, the recombinant plasmid would lack which of the following properties? A It would replicate autonomously. B) It would be resistant to the antibiotic ampicillin. C) It would possess at least two promoter sites. (D) It would be resistant to the antibiotic tetracycline. Pul Pod- ampr HIID EcoRI BamHI pBR322 (4363 bases) or Prull let Aval -Sall
Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' ? b) Assuming that the % GC is = 60%. O (2/10)4 (3/10)² O (3/10)4 (2/10)² O (1/6)4 O (1/4)6 O (6/10)4 (4/10)2
QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration. Agarose gel concentration: Time: Voltage:
The nucleosome assembly factor exclusively functions in DNA-dependent nucleosome assembly and binds to the PCNA (clamp) of DNA polymerase. (Capitalization and dashes/spaces are not critical.)
Question 3 In the polymerase chain reaction (A) conditions must be carefully controlled to prevent explosions. (B) reaction mixtures must be kept chilled at all times. C) it is possible to amplify small amounts of DNA without cloning. (D) primers should have a high G-C content and Tm at around 95°C

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