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Q44: Among the following, the least sensitive assay for measuring protein concentration is: A) Lowry assay B) Bradford assay C) BCA D) Waddell E) Biuret

Q44: Among the following, the least sensitive assay for measuring protein concentration is: A) Lowry assay B) Bradford assay C) BCA D) Waddell E) Biuret

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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please solve question 44

 

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Q42: The following describes Beer's law, except: A) The absorbance of light is proportional to the length of the light path B) The absorbance of light is proportional to the concentration C) The intensity of the transmitted light is higher than the initial intensity of the light D) There is a linear relationship between the concentration and the absorbance E) None of the above are correct Q43: The maximum absorption of methylene blue dye is at 664 nm, what is the absorbance of methylene blue at a concentration=5.0x106 mol/L if the path length=1 cm and € 664= 95000 L mol-¹ cm-¹? A) 0.475 B) 0.62 C) 0.25 D) 1 E) 0.019 Q44: Among the following, the least sensitive assay for measuring protein concentration is: A) Lowry assay B) Bradford ássay C) BCA D) Waddell E) Biuret Q45: The limitation of using UV absorption at 280 nm to determine protein concentration is: A) Complex assay and not easy to use B) It has very low sensitivity in detecting proteins (around 1-20 mg) C) Ideal for protein mixtures with variable content of aromatic amino acids D) Nucleic acids interfere with protein concentration measurement using this assay E) None of the above
Transcribed Image Text:Q42: The following describes Beer's law, except: A) The absorbance of light is proportional to the length of the light path B) The absorbance of light is proportional to the concentration C) The intensity of the transmitted light is higher than the initial intensity of the light D) There is a linear relationship between the concentration and the absorbance E) None of the above are correct Q43: The maximum absorption of methylene blue dye is at 664 nm, what is the absorbance of methylene blue at a concentration=5.0x106 mol/L if the path length=1 cm and € 664= 95000 L mol-¹ cm-¹? A) 0.475 B) 0.62 C) 0.25 D) 1 E) 0.019 Q44: Among the following, the least sensitive assay for measuring protein concentration is: A) Lowry assay B) Bradford ássay C) BCA D) Waddell E) Biuret Q45: The limitation of using UV absorption at 280 nm to determine protein concentration is: A) Complex assay and not easy to use B) It has very low sensitivity in detecting proteins (around 1-20 mg) C) Ideal for protein mixtures with variable content of aromatic amino acids D) Nucleic acids interfere with protein concentration measurement using this assay E) None of the above
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