Q3: When performing a gram stain, it is necessary to remove crystal violet stain from any gram negative bacteria on the slide. Microbiologists use a decolorizing solution to accomplish this. A common recipe is equal parts 95% ethanol and acetone.  How many mL of water would you need to add if you have 98% ethanol and want to end up with 460 mL of decolorizer? Report your answer to 2 decimal places and perform all calculations in a singlet step using Excel to avoid accruing rounding error.

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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When performing a gram stain, it is necessary to remove crystal violet stain from any gram negative bacteria on the slide. Microbiologists use a decolorizing solution to accomplish this. A common recipe is equal parts 95% ethanol and acetone. 

How many mL of water would you need to add if you have 98% ethanol and want to end up with 460 mL of decolorizer?

Report your answer to 2 decimal places and perform all calculations in a singlet step using Excel to avoid accruing rounding error.

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