Q3 In the laboratory, a flask containing 100 ml of culture medium X is inoculated with the microorganism Y. This was subsequently incubated at 37° C with shaking, and aliquots are collected periodically to determine the cell number by measuring the number of colony forming units (see lecture notes) and the number of CFU/ml recorded in the table below. Time (hours) 0 Cell number (CFU/ml) 2.05 X10% 1 2.04 10 2 3 4 2.01 10% 2.14 10 2.34 106 5 2.69 10% 6 3.80 10 7 5.25 10 8 9 1.07 7.41 106 10 10 1.51 107 11 1.82 107 12 1.99 10 13 2.04 10 14 1.91 10 15 2.02 10 Using this information calculate the specific growth rate (p). Q4 Estimate the length of the lag phase, specific growth rate u. doubling time to. and the yield coefficient Y from the following batch fermentation data. Time Biomass Substrate 3 (hr) (g/L) (GL) 0 1 0.15 4 168 18 5.5 3.0 50
Q3 In the laboratory, a flask containing 100 ml of culture medium X is inoculated with the microorganism Y. This was subsequently incubated at 37° C with shaking, and aliquots are collected periodically to determine the cell number by measuring the number of colony forming units (see lecture notes) and the number of CFU/ml recorded in the table below. Time (hours) 0 Cell number (CFU/ml) 2.05 X10% 1 2.04 10 2 3 4 2.01 10% 2.14 10 2.34 106 5 2.69 10% 6 3.80 10 7 5.25 10 8 9 1.07 7.41 106 10 10 1.51 107 11 1.82 107 12 1.99 10 13 2.04 10 14 1.91 10 15 2.02 10 Using this information calculate the specific growth rate (p). Q4 Estimate the length of the lag phase, specific growth rate u. doubling time to. and the yield coefficient Y from the following batch fermentation data. Time Biomass Substrate 3 (hr) (g/L) (GL) 0 1 0.15 4 168 18 5.5 3.0 50
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Q3 In the laboratory, a flask containing 100 ml of culture medium X is inoculated with
the microorganism Y. This was subsequently incubated at 37° C with shaking, and
aliquots are collected periodically to determine the cell number by measuring the
number of colony forming units (see lecture notes) and the number of CFU/ml
recorded in the table below.
Time (hours)
0
Cell number (CFU/ml)
2.05 X10%
1
2.04 10
2
3
4
2.01 10%
2.14 10
2.34 106
5
2.69 10%
6
3.80 10
7
5.25 10
8
9
1.07
7.41 106
10
10
1.51 107
11
1.82 107
12
1.99 10
13
2.04 10
14
1.91 10
15
2.02 10
Using this information calculate the specific growth rate (p).
Q4 Estimate the length of the lag phase, specific growth rate u. doubling time to. and
the yield coefficient Y from the following batch fermentation data.
Time Biomass Substrate
3
(hr)
(g/L)
(GL)
0
1
0.15
4
168
18
5.5
3.0
50
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