Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data:
Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data:
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast
extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add
glucose to both extracts at time zero, but to one (in red) you also add inorganic
phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated
by the two solutions over time and obtain the following data:
+ additional
Pi
+Pi
no Pi added
Time (min)
a) Why is more CO2 generated upon the addition of phosphate?
b) During fermentation, what would the ratio of CO2 generated to phosphate added
expected to be?
c) During fermentation, what would the ratio of CO2 generated to glucose
consumed expected to be?
d) You become worried that the flasks would burst open to the build up in gas
pressure. What other molecules could you add to the extract to slow down CO2
generation even in the presence of glucose and Pi?
CO, generated (mmoles)
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