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- Cells go to great length to correctmistakes in the processes of DNAreplication, transcription, splicing,and translation. Are there analogousstrategies to correct mistakes in theselection of which genes are to beexpressed in a given cell type? Couldthe great complexity of transcriptioninitiation in animals and plants reflectsuch a strategy?iestiðn Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following steps seemes to NOT help for the PIC formation? O TBP protein to recognize TATA box at promoter region O TFIIA to recognize the TAFS in TFIID O Enhancers to attract coactivators and activators O Silencers to attract repressor and corepressorsHow the transcription of one gene can be regulatedby the interplay of multiple promoter and enhancer elementsand the transcription factors ?
- Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription of heterochromatin (c) produce related but different proteins in different tissues (d) amplify genes to meet the requirement of high levels of a gene product (e) bind transcription factors to enhancers to activate transcriptionMechanisms that govern gene expression do not operate during ___ . a. transcription b. RNA processing c. translation d. knockoutsI. The retinoic acid receptor (RAR) is a transcription factor that is similar to steroid hormone receptors. Thesubstance (ligand) that binds to this receptor is retinoicacid. One of the genes whose transcription is activatedby retinoic acid binding to the receptor is myoD. Thediagram that follows shows a schematic view of theRAR proteins produced by genes into which one oftwo different 12-base double-stranded oligonucleotides had been inserted in the ORF. The insertion site(a–m) associated with each mutant protein is indicatedwith the appropriate letter on the polypeptide map.For constructs encoding proteins a–e, oligonucleotide 1(5′ TTAATTAATTAA 3′ read off either strand) wasinserted into the RAR gene. For constructs encoding proteins f–m, oligonucleotide 2 (5′ CCGGCCGGCCGG 3′)was inserted into the gene.NH2 f g h i j k l m COOHa b c d eThe wild-type RAR protein can both bind DNA and activate transcription weakly in the absence of retinoic acid(RA) and strongly in RA’s presence. Each…
- Enhancers contain binding sites for transcription factors that regulate transcription. are activators that act across insulator barriers. contain binding sites for the basal transcription apparatus. are necessary for transcription, but are not involved in regulating transcription.1. (a) Binding of transcription activator protein Gene is switched ON Gene is switched OFF Does NOT affect the gene expression (b) the RNA polymerase binds at the promoter region Gene is switched ON Gene is switched OFF Does NOT affect the gene expression") genes are atftected. PREDICT How might a mutation that affects a regulatory element, such as a promoter, transcription factor, or enhancer, affect the expression of a gene? Would the gene be expressed? If so, how might its expression change? Start Typing... DNA RNA polymerase gene enhancer TATA box promoter transcription factors
- Explain Molecular Mechanisms of Transcription Repression and Activation.e table below with the word that matches the definition in the right column. regulatory proteins (transcription factors) that speed up transcription by helping RNA polymerase bind to a promoter DNA sequences that indicate where transcription of a gene by RNA polymerase begins-typically located upstream from the 5C end Proteins bound to DNA that affect when and how fast a gene is transcribed These factors slow down/shut off transcription either by preventing access to promoters or by keeping RNA polymerase from moving along the DNA strand 3 Regulatory DNA sequénces located upstream or downstream from an associated gene that enhance the transcription rate of that gene when bound to specific proteins 3A sequence of DNA that when bound to a transcription factor blocks the inappropriate effect of an enhancer on a neighboring geneHow do the λ repressor and the cro protein affect the transcriptionfrom PR and PRM? Explain where these proteins are binding tocause their effects.