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Prokaryotes are all around us. What experiment can be Developed to visualize these microbes from an environmental sample.
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- You are working for the CDC studying outbreaks of foodborne infectious disease. Your latest case is an incidence of food poisoning in a fast food restaurant. 5 people have been hospitalized for food poisoning, so you are working with that hospital to identify the source of the outbreak. Hospital clinicians have taken samples from each of the 5 patients for culturing. Each bacterial culture grew one likely microbe, each labelled P1, P2, P3, P4, and P5. All 5 isolates appear to have the same colony morphology on the plates, and all appear to be Gram-negative rods 1 micron wide and 3 microns long under the microscope. In addition, all 5 isolates appear to perform the same on an API 20E test strip that tests bacteria for 20 biochemical abilities. No other testing has been done yet. How would you best describe your set of isolates at this moment? O Each isolate is the same strain, of a different species O Each isolate is a different strain, of different species O Each isolate is a different…Microbiologists have announced the discovery of over 30 new species of bacteria that thrive between the teeth and gums of humans. The bacteria could not be grown in the researcher's laboratories, nor were any of them ever observed via and kind of microscopy. If they couldn't culture them or see them, how could the researchers know they discovered a new species? If they couldn't examine the cells for the presence of a nucleus, how did they determine that the organisms were prokaryotes and not eukaryotes?Provide evidence in supporting or refuting the following statement: The cell, or cytoplasmic membrane, is a nonessential structure in bacteria because its function is replaced by the cell wall in these microbes. please provide minimum of 400 of words. Thank you!
- Nanobes may or may not actually be bacteria.True or false?Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution. Microbial populations grow at different rates based on temperature. One way to measure growth is by turbidity measurements. When bacteria multiply in a broth culture they turn the clear solution cloudy, or turbid. a) in your own words explain the proper steps to Inoculate 5°C sterile broth tube using aseptic techniqueProvide evidence in supporting or refuting the following statement: The cell, or cytoplasmic membrane, is a nonessential structure in bacteria because its function is replaced by the cell wall in these microbes. provide at least 400 of words
- What are the flaws ? Prokaryotes can live in many different environments. Anerobic organisms need oxygen to survive, while aerobic organisms can survive without oxygen. Autotrophs are organisms that can assemble high energy macromolecules for their energy. Chemotrophs are organisms that capture energy from light. Extremophiles are organisms that grow in extreme conditions like boiling water. Gram-positive bacteria have few layers of peptidoglycan, while gram-negative bacteria have many layers of peptidoglycan and are surrounded by an outer membrane. Harmful bacteria can cause illness like food poisoning. Helpful bacteria can be used in food production to make yogurt. The endosymbiotic theory states that some of the organelles in eukaryotes were once free living prokaryotes. This is supported by the mitochondria and cytoplasm since they have their own DNA and ribosomes. Harmful protists can cause diseases like malaria in humans and downy mildew in plants. Helpful protists can be…Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?
- The microbes discovered in Lake Whillans and Pitch Lake represented a few of the general categories of microbial life. Drag the images and/or descriptions to their corresponding class to test your understanding of the main types of microbes. Used by humans in the production of some antibiotics Ciliate Prokaryotic organisms used in genetic engineering due to their simple genetic material Photosynthetic organisms that may be used to produce alternative fuels Require electron microscopy for visualization Spores Bacilli Cysts in tissue Photosynthefic Bacteria Protozoans Fungi Viruses Helminths Algae Source: CDC Source: CDC Source: National Human Genome Reasearch Institute Source:Dr. Libero ajello/CDC ResetWhat is the difference between culture media and cultural environment? Are environmental conditions related to specific microbes? If so, which conditions for which type of microbes? If you were a field researcher collecting microbes in the wild, what kind of data would you take to make sure you know the exact environment the samples require in order to culture them in a lab?In lab, sterile technique is important for what reason? none of the answers are correct to ensure that we have not introduced outside microbial variables to prevent our samples from being contaminated with human samples all of the answers are correct to prevent us from getting sick with microbes