Problem 19.04. Gel Electrophoresis is an experimental procedure which can be used to separate DNA and/or RNA molecules by size. The molecules are placed in a gel then electrodes are added to create a potential difference across the gel. The molecules, which are charged, feel two forces, the electric force due to the presence of the electric field, and a drag force due to inter-molecular forces. The drag force is non-linear and difficult to model, so for experimental purposes the electrophoritic mobility µe = v/E of molecules is measured, which tells you the velocity v of a molecule for a given electric field strength E. 100. V are placed in a gel a distance Two electrodes with a potential difference of V = = V.s d 20.0 cm apart, and two molecules M₁ and M₂ with mobility µe,1 4.00 × 10-4 cm² and He,2 2.00 x 10-4 cm² are placed in a gel. (a) How long before a microscope with a resolution of R 200. mum can tell the molecules apart? (b) What is the maximum time the gel can run for before the faster molecule gets to the edge of the container? V.s = =
Problem 19.04. Gel Electrophoresis is an experimental procedure which can be used to separate DNA and/or RNA molecules by size. The molecules are placed in a gel then electrodes are added to create a potential difference across the gel. The molecules, which are charged, feel two forces, the electric force due to the presence of the electric field, and a drag force due to inter-molecular forces. The drag force is non-linear and difficult to model, so for experimental purposes the electrophoritic mobility µe = v/E of molecules is measured, which tells you the velocity v of a molecule for a given electric field strength E. 100. V are placed in a gel a distance Two electrodes with a potential difference of V = = V.s d 20.0 cm apart, and two molecules M₁ and M₂ with mobility µe,1 4.00 × 10-4 cm² and He,2 2.00 x 10-4 cm² are placed in a gel. (a) How long before a microscope with a resolution of R 200. mum can tell the molecules apart? (b) What is the maximum time the gel can run for before the faster molecule gets to the edge of the container? V.s = =
College Physics
11th Edition
ISBN:9781305952300
Author:Raymond A. Serway, Chris Vuille
Publisher:Raymond A. Serway, Chris Vuille
Chapter1: Units, Trigonometry. And Vectors
Section: Chapter Questions
Problem 1CQ: Estimate the order of magnitude of the length, in meters, of each of the following; (a) a mouse, (b)...
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