Preparation of buffers 1. Prepare 250ml of the following buffer solutions. Concentration Buffer solution pka Desired ph 0.50 M Acetate 4.70 5.0 Identify the weak acid and conjugate base of the components of each buffer. Compute the buffer components using the Henderson-Hasselbalch equation.

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Chapter1: Chemical Foundations
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-Buffer Preparation- 

 

Materials/Chemicals:
Volumetric flasks
Beakers
Acid-base indicators:
Serologic pipetters
Aspirators
Vials/test tubes
Cone. H3PO4 (85% w/w, sp gr. 1.70, MM 98)
Cone. HCL (37.3% w/w sp. Gr. 1.18, MM 36)
Glacial acetic acid (99.7% ww, sp gr. 1.05, MM
60)
NaOH pellets
NaH2PO4
Bromophenol Blue
Bromocresol green
Phenol red
Methyl red
Methyl orange
Phenolphthalein
Thymol Blue
рH pаper
pH meter
Weighing balance
Na2HPO4
Methodolody: (Include a short summary of the overall experimental plan you are going to run. You
may use flow chart or bullet form for this part. Sketches of any experimental set-ups used in the
experiment or the workup procedures).
Preparation of buffers
1. Prepare 250ml of the following buffer solutions.
Concentration
Buffer solution
pka
Desired ph
0.50 M
Acetate
4.70
5.0
Identify the weak acid and conjugate base of the components of each buffer. Compute the buffer
components using the Henderson-Hasselbalch equation.
Transcribed Image Text:Materials/Chemicals: Volumetric flasks Beakers Acid-base indicators: Serologic pipetters Aspirators Vials/test tubes Cone. H3PO4 (85% w/w, sp gr. 1.70, MM 98) Cone. HCL (37.3% w/w sp. Gr. 1.18, MM 36) Glacial acetic acid (99.7% ww, sp gr. 1.05, MM 60) NaOH pellets NaH2PO4 Bromophenol Blue Bromocresol green Phenol red Methyl red Methyl orange Phenolphthalein Thymol Blue рH pаper pH meter Weighing balance Na2HPO4 Methodolody: (Include a short summary of the overall experimental plan you are going to run. You may use flow chart or bullet form for this part. Sketches of any experimental set-ups used in the experiment or the workup procedures). Preparation of buffers 1. Prepare 250ml of the following buffer solutions. Concentration Buffer solution pka Desired ph 0.50 M Acetate 4.70 5.0 Identify the weak acid and conjugate base of the components of each buffer. Compute the buffer components using the Henderson-Hasselbalch equation.
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