Pre-Lab Assignment Title: Ziehl-Neelsen Acid-Fast Method: (replaces Kinyoun method in lab manual) *Due for each new lab - at the start of lab and before the lab/safety introduction. Use a new sheet for each different procedure/test. Pre-lab must be completed PRIOR to entrance to the lab. This is your ticket into the lab room. No exceptions will be made. Purpose: Procedure: List the steps in the procedure. 1.air dry bacteria on slide 2. HEAT FIX 3.make paper towel square over organism 4. Put over steam bath (use clothes pin; steam burns) 5. Saturate with carbol fuchsin 6. 8 minutes, keep wet with carbol fuchsin while steaming 7. bring back to bench on staining tray, remove paper 8. allow slide to cool on tray on bench 9. rinse with distilled water, tip on towel 10. decolorize with acid alcohol...until just runs clear, DROP by DROP watch carefully 11. rinse immediately with distilled water
Pre-Lab Assignment Title: Ziehl-Neelsen Acid-Fast Method: (replaces Kinyoun method in lab manual) *Due for each new lab - at the start of lab and before the lab/safety introduction. Use a new sheet for each different procedure/test. Pre-lab must be completed PRIOR to entrance to the lab. This is your ticket into the lab room. No exceptions will be made. Purpose: Procedure: List the steps in the procedure. 1.air dry bacteria on slide 2. HEAT FIX 3.make paper towel square over organism 4. Put over steam bath (use clothes pin; steam burns) 5. Saturate with carbol fuchsin 6. 8 minutes, keep wet with carbol fuchsin while steaming 7. bring back to bench on staining tray, remove paper 8. allow slide to cool on tray on bench 9. rinse with distilled water, tip on towel 10. decolorize with acid alcohol...until just runs clear, DROP by DROP watch carefully 11. rinse immediately with distilled water
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Pre-Lab Assignment Title: Ziehl-Neelsen Acid-Fast Method: (replaces Kinyoun method in lab manual)
*Due for each new lab - at the start of lab and before the lab/safety introduction. Use a new sheet for each different procedure/test.
Pre-lab must be completed PRIOR to entrance to the lab. This is your ticket into the lab room. No exceptions will be made.
Purpose:
Procedure:
List the steps in the
procedure.
Cultures needed:
*Use proper naming
and shorthand
notations
Changes to
Protocol:
Results:
*Describe what a
positive/negative
result will look like
Predicted Results/
Other:
List predicted results
for each organism
1.air dry bacteria on slide
2. HEAT FIX
3.make paper towel square over organism
4. Put over steam bath (use clothes pin; steam burns)
5. Saturate with carbol fuchsin
6. 8 minutes, keep wet with carbol fuchsin while steaming
7. bring back to bench on staining tray, remove paper
8. allow slide to cool on tray on bench
9. rinse with distilled water, tip on towel
10. decolorize with acid alcohol...until just runs clear, DROP by DROP watch carefully
11. rinse immediately with distilled water
12. counterstain with methylene blue 3 min
13. rinse with distilled water, tip on towel, blot dry, air dry to fully dry
14. no coverslip, use microscope, record shape, arrangement, acid-fast result
ylococcus Epidermidis, Mycobacterium Smegmatis
Acid-Fast will be performed using the ziehl-Neelsen
method.
Positive Result:
Brief Explanation:
Acid-fast bacteria pink orred
Staphylo
(72-h),
Negative Result:
Non-acid-fast bacteria blue
Bio 235 Pre lab Sheets May 31 2022.docx
Brief Explanation:
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