Please help with on the guide questions section below.  Thank you.

Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Chapter9: Acids, Bases, And Salts
Section: Chapter Questions
Problem 9.109E
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Please help with on the guide questions section below. 

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**Experiment 4: pH and Buffers**

**Materials Needed:**

- Red Cabbage
- Purified Water
- 5 Different Glasses/Jars
- Sprite (40 mL)
- Dropper/Syringe
- Baking Soda (2 teaspoons) NaHCO₃
- Lemon Juice/Calamansi Juice/Vinegar (choose one)
- Concentrated Lye (liquid sosa) Drainage Cleaner

**Procedure:**

1. **Prepare Cabbage Indicator:**
   - Boil red cabbage in 700 mL of water. Cut the leaves into small pieces to maximize the amount of indicator in the solution. This will serve as your pH indicator.

2. **Cool and Divide Solution:**
   - Let the solution cool and transfer 100 mL to each jar.

3. **Label and Prepare Containers:**
   - Label the containers A, B, C, and D. Add 1 teaspoon of baking soda and 20 mL of Sprite into containers B and C. Stir well.

4. **Test with Lemon Juice/Acid:**
   - In container A, add 1 mL dropwise of lemon juice or any acid. Stir well and observe the color change.
   - In container B, add 1 mL of lemon juice or any acid. Observe the changes in color. Add another 1 mL of lemon juice.

5. **Test with Concentrated Lye:**
   - In container D, add 1 mL of concentrated lye. Stir well and observe the color changes.
   - In container C, add 1 mL of concentrated lye. Stir well and observe the color changes.

**Observations:**

| Container | Observations after 1 mL of lemon juice is added | Observations after 1 mL of concentrated lye is added |
|-----------|------------------------------------------------|-----------------------------------------------------|
| A         | Color changed                                  | -------                                             |

This experiment demonstrates the effect of acids and bases on pH indicators using the natural pigment from red cabbage.
Transcribed Image Text:**Experiment 4: pH and Buffers** **Materials Needed:** - Red Cabbage - Purified Water - 5 Different Glasses/Jars - Sprite (40 mL) - Dropper/Syringe - Baking Soda (2 teaspoons) NaHCO₃ - Lemon Juice/Calamansi Juice/Vinegar (choose one) - Concentrated Lye (liquid sosa) Drainage Cleaner **Procedure:** 1. **Prepare Cabbage Indicator:** - Boil red cabbage in 700 mL of water. Cut the leaves into small pieces to maximize the amount of indicator in the solution. This will serve as your pH indicator. 2. **Cool and Divide Solution:** - Let the solution cool and transfer 100 mL to each jar. 3. **Label and Prepare Containers:** - Label the containers A, B, C, and D. Add 1 teaspoon of baking soda and 20 mL of Sprite into containers B and C. Stir well. 4. **Test with Lemon Juice/Acid:** - In container A, add 1 mL dropwise of lemon juice or any acid. Stir well and observe the color change. - In container B, add 1 mL of lemon juice or any acid. Observe the changes in color. Add another 1 mL of lemon juice. 5. **Test with Concentrated Lye:** - In container D, add 1 mL of concentrated lye. Stir well and observe the color changes. - In container C, add 1 mL of concentrated lye. Stir well and observe the color changes. **Observations:** | Container | Observations after 1 mL of lemon juice is added | Observations after 1 mL of concentrated lye is added | |-----------|------------------------------------------------|-----------------------------------------------------| | A | Color changed | ------- | This experiment demonstrates the effect of acids and bases on pH indicators using the natural pigment from red cabbage.
**Table Explanation**

The table describes the effects of different substances on the color change in containers A, B, C, and D after adding lemon juice and concentrated lye.

| **Container** | **Observations after 1 ml of substance of lemon juice is added** | **Observations after 1 ml of substance of concentrated lye is added** | **Conclusion**                        |
|---------------|--------------------------------------------------------|-------------------------------------------------|-------------------------------------------|
| A             | Color changed                                          | -------                                         | Color changed from blue to purple.        |
| B             | No changes                                             | -------                                         | Color didn’t change.                      |
| C             | -------                                                | Color changed                                   | Color changed from blue to yellow/dark brown. |
| D             | ------------                                           | Color changed                                   | Color changed from blue to yellow/dark brown. |

**Guide Questions**

1. **What caused the color changes in containers A, B, C, and D?**
2. **What is the effect of the sprite/baking soda mixture on the acidity and basicity of the mixture?**
   - When the sprite/baking soda was added, it made the mixture neutral.
3. **What are buffers and why is it important in biochemistry?**
   - Buffers
4. **Describe the Henderson-Hasselbalch equation and explain its use in making buffers.**
Transcribed Image Text:**Table Explanation** The table describes the effects of different substances on the color change in containers A, B, C, and D after adding lemon juice and concentrated lye. | **Container** | **Observations after 1 ml of substance of lemon juice is added** | **Observations after 1 ml of substance of concentrated lye is added** | **Conclusion** | |---------------|--------------------------------------------------------|-------------------------------------------------|-------------------------------------------| | A | Color changed | ------- | Color changed from blue to purple. | | B | No changes | ------- | Color didn’t change. | | C | ------- | Color changed | Color changed from blue to yellow/dark brown. | | D | ------------ | Color changed | Color changed from blue to yellow/dark brown. | **Guide Questions** 1. **What caused the color changes in containers A, B, C, and D?** 2. **What is the effect of the sprite/baking soda mixture on the acidity and basicity of the mixture?** - When the sprite/baking soda was added, it made the mixture neutral. 3. **What are buffers and why is it important in biochemistry?** - Buffers 4. **Describe the Henderson-Hasselbalch equation and explain its use in making buffers.**
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