Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter30: Nutrition
Section: Chapter Questions
Problem 30.43P
Related questions
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Please check if my answers and process are correct
![If your stock concentration of ampicillin is 500 mg/ml, how much would you add to 500 ml of
Luria Agar (LA) to have a final ampicillin concentration of 100 ug/ml? Use C1V1=C2V2. Show
your work.
1000 ug/mL.
500 mg/mL (
= 500,000 ug/mL
1 mg/mL
500,000 ug/mL (V1) = 100 ug/mL (500 mL)
V1 = 0.1 mL or 100 uL
If each plate contained ~20 ml of LA (agar form of lysogeny broth), what is the approximate
amount of ampicillin (grams) in each plate? Use the information from the previous question to
help you. Show your work.
100 ug/mL x 20 mL = 2,000 ug/mL = 2 mg/mL
How much of the same ampicillin stock [100 mg/ml] would you add to 3 ml of LB (lysogeny
broth) to have a final concentration of 100 ug/ml? Use C1V1=C2V2. Show your work.
1000 ug/mL,
100 mg/mL(
100,000 ug/mL
=
1 mg/mL
100,000 ug/mL (V1) = 100 ug/mL (3mL)
V1 = 0.003 mL or 3 uL](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F43abe65f-7e5f-4ca7-a91c-ab097f3acb86%2Fd79f229d-6a2a-43b1-823a-7c4b426aeec8%2Fl3rcqz9_processed.png&w=3840&q=75)
Transcribed Image Text:If your stock concentration of ampicillin is 500 mg/ml, how much would you add to 500 ml of
Luria Agar (LA) to have a final ampicillin concentration of 100 ug/ml? Use C1V1=C2V2. Show
your work.
1000 ug/mL.
500 mg/mL (
= 500,000 ug/mL
1 mg/mL
500,000 ug/mL (V1) = 100 ug/mL (500 mL)
V1 = 0.1 mL or 100 uL
If each plate contained ~20 ml of LA (agar form of lysogeny broth), what is the approximate
amount of ampicillin (grams) in each plate? Use the information from the previous question to
help you. Show your work.
100 ug/mL x 20 mL = 2,000 ug/mL = 2 mg/mL
How much of the same ampicillin stock [100 mg/ml] would you add to 3 ml of LB (lysogeny
broth) to have a final concentration of 100 ug/ml? Use C1V1=C2V2. Show your work.
1000 ug/mL,
100 mg/mL(
100,000 ug/mL
=
1 mg/mL
100,000 ug/mL (V1) = 100 ug/mL (3mL)
V1 = 0.003 mL or 3 uL
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