Please check if my answers and process are correct If your stock concentration of ampicillin is 500 mg/ml, how much would you add to 500 ml ofLuria Agar (LA) to have a final ampicillin concentration of 100 ug/ml? Use C1V1=C2V2. Showyour work.1000 ug/mL.500 mg/mL (= 500,000 ug/mL1 mg/mL500,000 ug/mL (V1) = 100 ug/mL (500 mL)V1 = 0.1 mL or 100 uLIf each plate contained ~20 ml of LA (agar form of lysogeny broth), what is the approximateamount of ampicillin (grams) in each plate? Use the information from the previous question tohelp you. Show your work.100 ug/mL x 20 mL = 2,000 ug/mL = 2 mg/mLHow much of the same ampicillin stock [100 mg/ml] would you add to 3 ml of LB (lysogenybroth) to have a final concentration of 100 ug/ml? Use C1V1=C2V2. Show your work.1000 ug/mL,100 mg/mL(100,000 ug/mL=1 mg/mL100,000 ug/mL (V1) = 100 ug/mL (3mL)V1 = 0.003 mL or 3 uL

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Chemistry

Please check if my answers and process are correct

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter30: Nutrition

Section: Chapter Questions

Problem 30.3P: If sodium benzoate, a food preservative, is excreted as such and if calcium propionate, another food...

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Question

Please check if my answers and process are correct

If your stock concentration of ampicillin is 500 mg/ml, how much would you add to 500 ml of
Luria Agar (LA) to have a final ampicillin concentration of 100 ug/ml? Use C1V1=C2V2. Show
your work.
1000 ug/mL.
500 mg/mL (
= 500,000 ug/mL
1 mg/mL
500,000 ug/mL (V1) = 100 ug/mL (500 mL)
V1 = 0.1 mL or 100 uL
If each plate contained ~20 ml of LA (agar form of lysogeny broth), what is the approximate
amount of ampicillin (grams) in each plate? Use the information from the previous question to
help you. Show your work.
100 ug/mL x 20 mL = 2,000 ug/mL = 2 mg/mL
How much of the same ampicillin stock [100 mg/ml] would you add to 3 ml of LB (lysogeny
broth) to have a final concentration of 100 ug/ml? Use C1V1=C2V2. Show your work.
1000 ug/mL,
100 mg/mL(
100,000 ug/mL
=
1 mg/mL
100,000 ug/mL (V1) = 100 ug/mL (3mL)
V1 = 0.003 mL or 3 uL

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