Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC) with a C18 stationary phase and the mobile phase buffered at pH = 2. The neutral species of each compound is shown. nicotine, PK₂1 = 3.15, PK₂2-7.85 N CH3 acetophenone CH3 Elutes first OH salicylic acid pK₂ = 2.97 OH

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Answer bank: salicylic acid, nicotine and acetophenone

Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography
(RPLC) with a C18 stationary phase and the mobile phase buffered at pH = 2. The neutral species of each compound is shown.
O
of a d
CH 3
OH
N
N
CH3
OH
acetophenone
nicotine,
pKa1 = 3.15,
pK₂2=7.85
Elutes first
salicylic acid
pka = 2.97
Transcribed Image Text:Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC) with a C18 stationary phase and the mobile phase buffered at pH = 2. The neutral species of each compound is shown. O of a d CH 3 OH N N CH3 OH acetophenone nicotine, pKa1 = 3.15, pK₂2=7.85 Elutes first salicylic acid pka = 2.97
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