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Picture this: You are on the verge of completing a key experiment for your PhD dissertatic project... and you realize you are running short on cell culture medium. To prepare a new 500 mL bottle, you are given the following recipe: Please complete the table by filling in the needed volumes to achieve the IMDM with 10% heat inactivated fetal bovine serum (HI FBS): IMDM 500 mL HI FBS (100 %) A mL 2 mM L-glutamine (200 mM) B mL 1,000 units-mg Pen/Strep C mL (10,000 units Pen/Strep per mL) *Please note: stock concentrations are indicated in blue and desired in black. A = mL B = mL ml

Picture this: You are on the verge of completing a key experiment for your PhD dissertatic project... and you realize you are running short on cell culture medium. To prepare a new 500 mL bottle, you are given the following recipe: Please complete the table by filling in the needed volumes to achieve the IMDM with 10% heat inactivated fetal bovine serum (HI FBS): IMDM 500 mL HI FBS (100 %) A mL 2 mM L-glutamine (200 mM) B mL 1,000 units-mg Pen/Strep C mL (10,000 units Pen/Strep per mL) *Please note: stock concentrations are indicated in blue and desired in black. A = mL B = mL ml

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Picture this: You are on the verge of completing a key experiment for your PhD dissertation project... and you realize you are running short on cell culture medium. To prepare a new 500 mL bottle, you are given the following recipe: Please complete the table by filling in the needed volumes to achieve the IMDM with 10% heat inactivated fetal bovine serum (HI FBS): IMDM 500 mL HI FBS (100 %) A mL 2 mM L-glutamine (200 mM) B mL 1,000 units-mg Pen/Strep C mL (10,000 units Pen/Strep per mL) *Please note: stock concentrations are indicated in blue and desired in black. A = mL B = mL C = mL
Transcribed Image Text:Picture this: You are on the verge of completing a key experiment for your PhD dissertation project... and you realize you are running short on cell culture medium. To prepare a new 500 mL bottle, you are given the following recipe: Please complete the table by filling in the needed volumes to achieve the IMDM with 10% heat inactivated fetal bovine serum (HI FBS): IMDM 500 mL HI FBS (100 %) A mL 2 mM L-glutamine (200 mM) B mL 1,000 units-mg Pen/Strep C mL (10,000 units Pen/Strep per mL) *Please note: stock concentrations are indicated in blue and desired in black. A = mL B = mL C = mL
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