Phenobarbital was introduced in 1912 for the treatment of epilepsy. It is an anti-convulsant and sedative-hypnotic drug and used to control seizures. The measurement of phenobarbital is by fluorescence method. A 5.00 mL aliquots of an unknown containing phenobarbital were delivered to 50.00 mL volumetric flasks. The following volumes of a standard solution of phenobarbital, 2.00ug/mL, were then introduced to the volumetric flasks before diluting to volume with the corresponding signals on a fluorometer equipment. Find the concentration of phenobarbital in the original unknown sample.

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Phenobarbital was introduced in 1912 for the treatment of epilepsy. It is an anti-convulsant and sedative-hypnotic drug and used to control seizures. The measurement of phenobarbital is by fluorescence method. A 5.00 mL aliquots of an unknown containing

phenobarbital were delivered to 50.00 mL volumetric flasks. The following volumes of a standard solution of phenobarbital, 2.00ug/mL, were then introduced to the volumetric flasks before diluting to volume with the corresponding signals on a fluorometer equipment. Find the concentration of phenobarbital in the original unknown sample.

 

:concentration of phenobarbital in the original unknown sample.
Volume of Standard Fluorescence
Phenobarbital (2.00 | Intensity, a.u.
ug/mL) added, mL
0.00
3.26
0.50
4.80
1.00
6.41
1.50
8.02
2.00
9.56
Transcribed Image Text::concentration of phenobarbital in the original unknown sample. Volume of Standard Fluorescence Phenobarbital (2.00 | Intensity, a.u. ug/mL) added, mL 0.00 3.26 0.50 4.80 1.00 6.41 1.50 8.02 2.00 9.56
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