Perform the calculations to prepare 100 mL of 0.02 M Phosphate buffer pH 9 using the stock solutions 1) 0.5 M sodium Phosphate Monobasic 2) 0.4 M sodium Dibasic Must show all steps • Log molar ratio ( base/acid)

Perform the calculations to prepare 100 mL of 0.02 M Phosphate buffer pH 9 using the stock solutions 1) 0.5 M sodium Phosphate Monobasic 2) 0.4 M sodium Dibasic Must show all steps • Log molar ratio ( base/acid)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Bromothymol blue (MWt = 624 g/mole, weak acid, pka 7.1) diluted solution is prepared by diluting 2.5
mL of 0.04 % w/v Bromothymol blue stock solution to final volume of 10 mL using 0.02 M Phosphate
buffer pH 9 ( pka 7.2).
Perform the calculations to prepare 100 ml of 0.02 M Phosphate buffer pH 9 using the stock solutions
1) 0.5 M sodium Phosphate Monobasic 2) 0.4 M sodium Dibasic
Must show all steps
• Log molar ratio ( base/acid)
• Molar ratio ( base/acid)
• Molarity of sodium Phosphate Monobasic (weak acid) in 0.02 M Phosphate buffer pH 9.
• Molarity of sodium Phosphate Dibasic (weak base) in 0.02 M Phosphate buffer pH 9.
• Volume of
o 0.5 M sodium Phosphate Monobasic
o 0.4 M sodium Dibasic
o Water (diluent)

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