Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:5. Describe in vivo steps of insulin expression in pancreas? Using the diagram below describe the
elements of insulin expression vectors. How many genes each vector has? How many different
proteins each plasmid expresses? Why do you need a gal coding region in each plasmid? Do you
have a stop codon between gal and insulin parts? How do you separate the gal part from insulin
part A or insulin part B?
B-galactosidase
gene
Promoter
Antibiotic
resistance
gene
neun gene
A subunit
0&
1
Promoter.
Antibiotic
B-galactosidase/rouin A
Ausion protein
Purity the fusion
protors
Dioufide
bridge Active insulin
Chemically remove insulin
polypeptides from the
B-galactosidase proteins
B-galactosidan
gone
Oa
→W-N
-insulin gene
Baubunt
Combine the
polypeptides
to produce
Aunctional insun
of the
polyende
highly ped gane
fo
Tracow
te meewe
and
B-galactosidaseroun B
fusion protein
gric
The inthote
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