Oxidation Fermentation (OF) Glucose utilization: O, F, or O-F or F’ only: __________________________

Human Anatomy & Physiology (11th Edition)
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Oxidation Fermentation (OF)

Glucose utilization: O, F, or O-F or F’ only: __________________________

**O/F Tube Result:**

The image displays two test tubes containing a yellow liquid with orange discoloration indicative of results from an Oxidation-Fermentation (O/F) test. The O/F test is primarily used to determine the oxidative and fermentative properties of microorganisms, especially in glucose metabolism. 

- **Left Test Tube (Oxygen Use or Open Tube):** This tube is typically exposed to air (aerobic conditions). The yellow color extending throughout the medium suggests that the microorganism is capable of breaking down the carbohydrate (glucose) oxidatively. The presence of yellow coloration at both the surface and throughout the tube represents oxidation.

- **Right Test Tube (Sealed or Closed Tube):** This tube is often sealed with a layer of oil or paraffin to create anaerobic conditions. The yellow discoloration at the surface extending throughout the tube indicates that the microorganism can ferment glucose in the absence of oxygen.

**Conclusion:**
The image shows positive results for both oxidative and fermentative metabolism of glucose. The yellow coloration in both tubes indicates that the microorganism can metabolize glucose under both aerobic and anaerobic conditions.

This test is vital in microbiology for identifying and characterizing bacteria, specifically their metabolic processes.
Transcribed Image Text:**O/F Tube Result:** The image displays two test tubes containing a yellow liquid with orange discoloration indicative of results from an Oxidation-Fermentation (O/F) test. The O/F test is primarily used to determine the oxidative and fermentative properties of microorganisms, especially in glucose metabolism. - **Left Test Tube (Oxygen Use or Open Tube):** This tube is typically exposed to air (aerobic conditions). The yellow color extending throughout the medium suggests that the microorganism is capable of breaking down the carbohydrate (glucose) oxidatively. The presence of yellow coloration at both the surface and throughout the tube represents oxidation. - **Right Test Tube (Sealed or Closed Tube):** This tube is often sealed with a layer of oil or paraffin to create anaerobic conditions. The yellow discoloration at the surface extending throughout the tube indicates that the microorganism can ferment glucose in the absence of oxygen. **Conclusion:** The image shows positive results for both oxidative and fermentative metabolism of glucose. The yellow coloration in both tubes indicates that the microorganism can metabolize glucose under both aerobic and anaerobic conditions. This test is vital in microbiology for identifying and characterizing bacteria, specifically their metabolic processes.
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