our team is asked to make up a 200mL agarose gel, 1%, in 1XTAE containing 0.5ug/mL of thidium bromide. You have the following stock reagents: 50XTAE, 10mg/mL ethidium romide, powder agarose and deionized water. Please calculate the following: 1. Amount of garose, 2. Volume of 50X TAE, 3. Volume of 10mg/mL ethidium bromide, 4. Volume of dei vater

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Chapter1: Chemical Foundations
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Your team is asked to make up a 200mL agarose gel, 1%, in 1XTAE containing 0.5ug/mL of
ethidium bromide. You have the following stock reagents: 50XTAE, 10mg/mL ethidium
bromide, powder agarose and deionized water. Please calculate the following: 1. Amount of
agarose, 2. Volume of 50X TAE, 3. Volume of 10mg/mL ethidium bromide, 4. Volume of dei
water
Transcribed Image Text:Your team is asked to make up a 200mL agarose gel, 1%, in 1XTAE containing 0.5ug/mL of ethidium bromide. You have the following stock reagents: 50XTAE, 10mg/mL ethidium bromide, powder agarose and deionized water. Please calculate the following: 1. Amount of agarose, 2. Volume of 50X TAE, 3. Volume of 10mg/mL ethidium bromide, 4. Volume of dei water
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