Your colleague has obtained a sample of muscle phosphorylase that is known to be relatively inactive. She has approached you for advice on how to set up an appropriate assay. She has the following items available, not all of which are appropriate for this study. Help her out by selecting the items that she should use and what their purpose is in the assay, and then explain why each of the other items would not be useful.1. 100 μM AMP2. 1.0 mM ATP3. 100 μM glucose4. 10 mM glucose 6-phosphate5. Unbranched glycogen6. Branched glycogen7. 50 mM HEPES buffer, pH 7.58. 50 mM potassium phosphate buffer, pH 7.5Write out a short explanation for each of the above items, and upload your answers by the due date.

muscle phosphorylase also called glycogen phosphorylase.

Ore of vr coleap has ainedaample of mule phohoryethkown to be reutely inace Sehas proched you tor adice on how to set upan ropriate y. Se has the floing item alae, not aof whh wepprriate for thi stuty. Hep her ot by wlecting the tems that he hod ueand what the prposeinthe H andthen eplain why ech of the her item wonet beet 1. 100 MAMP 2. 100 M GTP 3. 100 e

Ore of vr coleap has ainedaample of mule phohoryethkown to be reutely inace Sehas proched you tor adice on how to set upan ropriate y. Se has the floing item alae, not aof whh wepprriate for thi stuty. Hep her ot by wlecting the tems that he hod ueand what the prposeinthe H andthen eplain why ech of the her item wonet beet 1. 100 MAMP 2. 100 M GTP 3. 100 e

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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