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One of your classmates is planning to study endocytosis at neuronal synapses for their senior thesis. He is interested in what type of molecules neurons endocytose. They propose to use a cell line mutated in the microtubule motor kinesin to keep endocytic vesicles clustered at the synapse where they can be isolated into synaptosomes through differential centrifugation. The classmate shows you protocols for growing neurons in culture and one that demonstrates the feasibility of isolating synaptosomes. But you know there is something else wrong with this proposal that will keep it from succeeding. What is it?

One of your classmates is planning to study endocytosis at neuronal synapses for their senior thesis. He is interested in what type of molecules neurons endocytose. They propose to use a cell line mutated in the microtubule motor kinesin to keep endocytic vesicles clustered at the synapse where they can be isolated into synaptosomes through differential centrifugation. The classmate shows you protocols for growing neurons in culture and one that demonstrates the feasibility of isolating synaptosomes. But you know there is something else wrong with this proposal that will keep it from succeeding. What is it?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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I do not understand what would be wrong with the proposal
One of your classmates is planning to study endocytosis at neuronal synapses for their senior thesis. He is interested in what type of molecules neurons endocytose. They propose to use a cell line mutated in the microtubule motor kinesin to keep endocytic vesicles clustered at the synapse where they can be isolated into synaptosomes through differential centrifugation. The classmate shows you protocols for growing neurons in culture and one that demonstrates the feasibility of isolating synaptosomes. But you know there is something else wrong with this proposal that will keep it from succeeding. What is it?
Transcribed Image Text:One of your classmates is planning to study endocytosis at neuronal synapses for their senior thesis. He is interested in what type of molecules neurons endocytose. They propose to use a cell line mutated in the microtubule motor kinesin to keep endocytic vesicles clustered at the synapse where they can be isolated into synaptosomes through differential centrifugation. The classmate shows you protocols for growing neurons in culture and one that demonstrates the feasibility of isolating synaptosomes. But you know there is something else wrong with this proposal that will keep it from succeeding. What is it?
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