One of the buffers that can be used for running an agarose gel is 1x TBE (tris borate EDTA) buffer. The recipe for 1x TBE is: • 0.13 M tris • 45 mM boric acid • 2.5 mM EDTA How will you make 1 L of this buffer from 1 M stock solutions of tris, boric acid, and EDTA? Put your answers in the blanks. To make 1 Lof this buffer, would need: Lof 1 M Tris ml of 1 M boric acid ml of 1 M EDTA Then, these will be topped up with distilled water in a volumetric flask up to the 1 L mark.

One of the buffers that can be used for running an agarose gel is 1x TBE (tris borate EDTA) buffer. The recipe for 1x TBE is: • 0.13 M tris • 45 mM boric acid • 2.5 mM EDTA How will you make 1 L of this buffer from 1 M stock solutions of tris, boric acid, and EDTA? Put your answers in the blanks. To make 1 Lof this buffer, would need: Lof 1 M Tris ml of 1 M boric acid ml of 1 M EDTA Then, these will be topped up with distilled water in a volumetric flask up to the 1 L mark.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

The Ksp for Zn(OH)2 is 5.0 x 10-17. Determine the solubility (in g/L) of Zn(OH)2 in a. Pure water: pH 7 Zn(OH)2 = ____________ b. A buffer solution with a pH of 11.50. pH 11.50 Zn(OH)2 = ____________
How can we answer these questions?
How to determine the pH of a buffer created by adding 2.057 M Acetic Acid to 3.100 M Sodium Acetate with the given pKa of 2.55?
Consider the list of acids and their pKa values attached What mass ratio of sodium salt to acid is needed to prepare this buffer?
Which of the following would make the best buffer with the highest "buffer capacity"? 100 mM NH4+ and 5 mM NaOH 100 mM NH4+ and 10 mM NaOH 100 mM NH4+ and 25 mM NaOH 100 mM NH4+ and 50 mM NaOH 100 mM NH4+ and 100 mM NaOH
8) What weight of sodium carbonate (Na2CO3) and sodium bicarbonate (NaHCO3) are required to make 100 mls of a 0.1 M buffer, pH 9.8 (pKa1of H2CO3= 6.1, pKa2= 10.3, MW of Na2CO3= 106 g/mol, MW of NaHCO3= 84 g/mol)?
A new amino acid has 2 carboxyl groups. the pka's of these groups are 2.18 and 5.02. The amino acid has one amino group with a pka of 9.98. a) What is the pI of the amino acid? b) The number of end points I expect to see on a titration curve is ___ and the first buffer region would be centered around a pH of ___. The total number of buffer regions is ___. c) What is the pka of the side chain?
A buffer contains 0.040 mol of lactic acid (pK₁ = 3.86) and 0.065 mol of sodium lactate per liter. H₂C. OH Lactic acid OH Calculate the pH of the buffer. H₂C OH Sodium lactate Calculate the change in pH after adding 7.0 mL of 0.30 M HCl to 1.0 L of the buffer. O Na+ Calculate the change in pH after adding 7.0 mL of 0.30 M HCl to 1.0 L of pure water. buffer pH: 4.07 buffer pH change: water pH change: 0.4 Incorrect 3.15 Incorrect units units
BUFFERS, HYDROLYSIS OF SALTS 1. A 0.0250 M Ca(X)2 has a pH of 8.049. Ca(X)2 is composed of a Ca2+ cation and an unknown anion X™, which undergoes hydrolysis. A. Write the balanced dissociation reaction of Ca(X)2 into its ions. Write your answer on your solution sheet B. Write the balanced hydrolysis reaction of the unknown anion, X. Write your answer on your solution sheet C. Calculate the Kh or Kp of X. D. Given the following Ką values of different weak acids, determine the identity of X. Weak acids Ka HIO 2.00 x 10-11 HCN 6.17 x 10-10 CH3COOH 1.80 x 10-5 HNO2 4.00 x 10-4
None
35. The pH of a boric acid (Ka=5.8x10-10) buffer formed by the combination of 0.15 M boric acid that is also 0.10 M sodium borate is a. 9.42 b. 6.12 c. 9.06 d. 9.24
A buffer which contains 0.50 mol pyruvic acid and 0.45 mol sodium pyruvate dissolved in 1.0 L of water has a pH of 2.34. Assuming no significant changes to volume, calculate the pH when 0.10 mol of NaOH is added to the buffer. (The pk of pyruvic acid is 2.39) A. 2.34 B. 13.00 C. 2.15 D. 2.52 E. How do I do this? [base] pH=pKa + log. [acid]