On this casein plate, which organism(s) produce casease? This image shows a petri dish divided into three sections, labeled A, B, and C. Each section contains a separate bacterial culture, distinguished by color and growth patterns:- **Section A**: The agar appears clear, with a slight yellow hue, indicating minimal bacterial growth or the presence of a non-pigmented strain.- **Section B**: There is a noticeable green bacterial colony, suggesting the presence of a pigmented strain, possibly Pseudomonas aeruginosa, which is known to produce pyocyanin, a green-blue pigment.- **Section C**: A red bacterial colony is present, indicating a pigmented bacterium, potentially Serratia marcescens, known for its red pigment, prodigiosin.The dish is divided by lines into distinct areas to separate the bacterial growth, allowing for the observation and analysis of different bacterial types and their pigments for educational purposes.

There are some important terms which should be kept in mind. Nutrients are the substance used in biosynthesis and energy production and required for bacterial growth. All bacteria required macro and micronutrients. Any bacterium to be propagated required an environment for its growth so , a culture media which is a solid and liquid medium that contain all the nutrients required for its growth is developed with different purpose and uses , mainly used for identification of bacteria. Casein is the phosphoproteins found in milk eg cow milk, sheep milk buffalo milk. Casein act as good substrate for the culture of bacterias eg B. cereus. most of the microorganism have the ability to degrade the casein protein by producing an enzyme proteinase or caseinase. Main procedure is inoculate the organism on plate , incubate the plate and examine it for the presenc and absence of growth.Result of the above question are as follows. B and C of the petri plate produces casein and Casein act as good substrate for the culture of bacteria s eg B. cereus. We see some clearing around the colony that means the casein has undergone hydrolysis, this activity is seen in B and C sections of the petri plate. therefore B and C give the positive test and A give the negative test.