Nitrite is commonly determined by a colorimetric procedure using a reaction called the Griess reaction. In this reaction, the sample containing nitrite is reacted with sulfanilimide and N-(1-Napthyl) ethylenediamine to form a colored species that absorbs at 550 nm. Using an utomated flow analysis instrument, the following results were obtained for standard solutions of nitrite and for a sample containing an unknown amount: Solution Absorbance at 550 nm
Nitrite is commonly determined by a colorimetric procedure using a reaction called the Griess reaction. In this reaction, the sample containing nitrite is reacted with sulfanilimide and N-(1-Napthyl) ethylenediamine to form a colored species that absorbs at 550 nm. Using an utomated flow analysis instrument, the following results were obtained for standard solutions of nitrite and for a sample containing an unknown amount:
Solution Absorbance at 550 nm
2.00 μM 0.065
6.00 μM 0.205
10.00 μM 0.338
14.00 μM 0.474
18.00 μM 0.598
Unknown 0.402
(a) Find the slope, intercept, and standard deviation of the calibration curve.
(b) Construct the calibration curve.
(c) Determine the concentration of nitrite in the sample.
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