NALYSIS OF URINE 1.Urea – to 10 drops of urine, add 5 drops of conc. NaOH and 2 drops of bromine water. Note evolution of gas. 2. Uric acid - place 5 ml urine in a beaker and add 1 ml conc. HCl. Stir well and set aside until the next lab period. Describe the appearance of crystals deposited on the sides of the beaker. Dissolve a few crystals of uric acid in 2 ml 5% Na 2 CO 3 solution. Add 2ml Benedicts reagent and heat in a boiling water bath for 15 minutes. Observe formation of precipitate. 3. Creatinine – to 10 drops urine and 5 drops of sat. Picric acid. Drop red litmus paper to the mixture and make alkaline with 10% NaOH until color of litmus paper changes to blue. Observe color.
NALYSIS OF URINE 1.Urea – to 10 drops of urine, add 5 drops of conc. NaOH and 2 drops of bromine water. Note evolution of gas. 2. Uric acid - place 5 ml urine in a beaker and add 1 ml conc. HCl. Stir well and set aside until the next lab period. Describe the appearance of crystals deposited on the sides of the beaker. Dissolve a few crystals of uric acid in 2 ml 5% Na 2 CO 3 solution. Add 2ml Benedicts reagent and heat in a boiling water bath for 15 minutes. Observe formation of precipitate. 3. Creatinine – to 10 drops urine and 5 drops of sat. Picric acid. Drop red litmus paper to the mixture and make alkaline with 10% NaOH until color of litmus paper changes to blue. Observe color.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
ANALYSIS OF URINE
1.Urea – to 10 drops of urine, add 5 drops of conc. NaOH and 2 drops of
bromine water. Note evolution of gas.
2. Uric acid - place 5 ml urine in a beaker and add 1 ml conc. HCl. Stir well and
set aside until the next lab period. Describe the appearance of crystals
deposited on the sides of the beaker.
Dissolve a few crystals of uric acid in 2 ml 5% Na 2 CO 3 solution. Add 2ml
Benedicts reagent and heat in a boiling water bath for 15 minutes. Observe
formation of precipitate.
3. Creatinine – to 10 drops urine and 5 drops of sat. Picric acid. Drop red litmus
paper to the mixture and make alkaline with 10% NaOH until color of litmus
paper changes to blue. Observe color.
Transcribed Image Text:Result
C. Organic constituents
1.
Urea
2.
Uric acid
3.
Creatinine
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