**UV Spectroscopy and the Beer-Lambert Law in Determining DNA Weight**The weight of proteins or nucleic acids in solution is often determined by UV spectroscopy using the Beer-Lambert law. For example, the molar absorptivity, \( \varepsilon \), of double-stranded DNA at 260 nm is 6670 M\(^{-1}\)cm\(^{-1}\). The formula weight of the repeating unit in DNA (650 Daltons) can be used as the molecular weight.**Calculation Problem:**What is the weight of DNA in 4.0 mL of aqueous buffer if the absorbance, measured in a 1-cm cuvette, is 0.35?**Instruction:**Choose the correct value from the drop-down list provided.**Input:**Weight of DNA: [Dropdown options] gram*Note: Ensure to apply the Beer-Lambert law for accurate calculations, considering path length and concentration values appropriately.*

You have given the absorbance and molar absorptivity, then find the concentration of solution using…

molar absorptivity, ɛ, of double-stranded The weight of proteins or nucleic acids in solution is often determined by UV spectroscopy using the Beer-Lambert law. For example, DNA at 260 nm is 6670 M'cm-'. The formula weight of the repeating unit in DNA (650 Daltons) can be used as the molecular weight. What is the weight of DNA in 4.0 mL of aqueous buffer if the absorbance, measured in a 1-cm cuvette, is 0.35? Choose the correct value from the drop-down list provided. Weight of DNA: v gram

molar absorptivity, ɛ, of double-stranded The weight of proteins or nucleic acids in solution is often determined by UV spectroscopy using the Beer-Lambert law. For example, DNA at 260 nm is 6670 M'cm-'. The formula weight of the repeating unit in DNA (650 Daltons) can be used as the molecular weight. What is the weight of DNA in 4.0 mL of aqueous buffer if the absorbance, measured in a 1-cm cuvette, is 0.35? Choose the correct value from the drop-down list provided. Weight of DNA: v gram

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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