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Population Growth
R and K Selection
R and K selection are concepts in ecology used to describe traits in the fluctuation of a population or population dynamics. For example, they describe the life-association traits between parent and offspring, such as quantity or number of young ones born at a time, quality of parental care, the age to maturity, and reproductive effort.
could you please plot the semilog graph of the Growth Curve and calculate the doubling time of the attached data?
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- For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.For a microbial cell to grow at its maximum specific growth rate, which of the following conditions shall be matched? O S = (1/2)Ks O S = Ks O S >> Ks OS <use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30(b) A Food material containing Bacillus stearothermophilus PS1518 as an indicator organism ts subjected to heat sterilization at 121 C. Calculate the time required to reduce the organism to one tenth of the original number. Fo value for the organism is 4 minutes and the decimal reduction time , D, at 116°C is 40 minutes. Assume operation is at constant temperature of 121°C. HINT Fo = -To 10 dt Where Fo = equivalent exposure time at 121°C of the actual exposure time at a variable temperature To = the reference temperature =121 C, z=10 = number ofC necessary for10fold increase in FAY AY * 00 HI T 00 2 3. ment10: Connect Regis.. M https://Ims.mh.. nemical Content of Media i Saved Help Save & Exit Chec Chemically defined medium Complex medium 2 Enriched medium Fastidious Heterotroph 9 Autotroph Match each of the options above to the items below. Uses inorganic carbon as a carbon source for building its own organic molecules. A type of organism that has complex nutrient requirements and requires a nutritionally dense environment. A media containing extracts from other other organisms. A medium with specifically added nutrients to enhance the growth of fastidious organisms. An organism that uses organic carbon as its carbon source. A medium of which the exact chemical composition is known. MacBook Air DD F7 000 000 F2 F4 F5 F8 %23 2$ 4. 9- } 1 Y | 7.6.0 EXPECTED RESULT FOR BACTERIAL GROWTH CURVE Table 1 Culture 1,0ptical Density (OD 550) 0.07 0.07 Sampling Time, Minutes 30 60 0.08 90 0.10 120 0.13 150 0.42 180 0.78 0.92 210 240 1.02 270 1.07 300 1.17 330 1.22 360 1.27 390 420 1.27 1.28 Please plot a graph culture 1, OD 550 versus sampling time, minutes. Then discuss a phases of bacterial growth curve completely.If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationA bacteria colony's radius is measured as a function of time. t r(t) 0 1.01 0.1 1.031 Estimate the rate of growth Ar/At at t = 0.1 Give two digits past the decimal point. 0.2 1.052 0.3 1.074 0.4 1.096Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.The following chart contains four facets. Each shows the amount of a substance in different conditions: • when the cells are adhering to a surface • when the cells are growing in suspension for different amounts of time There are 1 different glyphs shown in the frames. suspension growth suspension growth 1433B 1433E 6.000% 4.000% 16.000% 10.666% 2.000% 5.333% 0.000% 0.000% 1433G 1433Z 4.500% 6.000% 3.000% 4.000% 1.500% 2.000% 0.000% 0.000% adhesion growth O min * 10 min 30 min 60 min growth O min uoisaupe 10 min 30 min 60 minA culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this pleaseSEE MORE QUESTIONS