Measurement of Bacterial Growth @ 4°C Plot of Absorbance vs. Time (mins) -LB, pH 3.2 LB, pH 7.2 -LB, pH 9.2 1.2 1 0.8 0.6 0.4 0.2 15 30 45 60 75 90 105 120 Time (mins) Measurement of Bacterial Growth @ 37°C Plot of Absorbance vs. Time (mins) -LB, pH 3.2 -LB, pH 7.2 -LB, pH 9.2 0.8 0.6 0.4 0.2 15 30 45 60 75 90 105 120 Time (mins) Measurement of Bacterial Growth @ 45°C Plot of Absorbance vs. Time (mins). - LB, pH 3.2 - LB, pH 7.2 LB, pH 9.2 1.5 1 0.5 15 30 45 60 75 90 105 120 Time (mins) Absorbance Absorbance Absorbance 1.
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
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