Match the following items: [4]1. 540 nmA. Wavescan2. Quantity of light of a specific wavelength absorbed by a sampleB. Standard series3. Reference samples with a concentration rangeC. Absorbance4. Absorbance values at all wavelengthsD. Purple

The absorbance of a solution of a biological molecule is determined by Lambert-Beer law. The law…

Answered: Match the following items: [4] 1.…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You are told to analyze the purity of your product with TLC. Which of the following TLC plates would indicate a pure product? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a A TLC plate where the product lane has one spot with an Rf value that exactly matches that of the starting material. b A TLC plate where the product lane has one spot which has a lower Rf value than the starting material. с A TLC plate where the product lane has two spots, one with a higher Rf value than the starting material and one with a lower Rf value. d A TLC plate where the product lane has one spot which has a higher Rf than the starting material.
You have prepared the following serial dilution using tryptone broth:d1: 0.1ml culture + 9.9 ml brothd2: 300µl d1 + 4.7 ml brothd3: 0.2ml d2 + 9800 µl broth1) Write your dilutions as a rational number AND in scientific format? What was your total dilution (in scientific notation)?2) What was the original concentration of your culture if you spread 0.1 ml of d3 and counted360 cfu?
Food dye Initial concentration (uM) Initial volume (mL) Final concentration (µM) Final volume (mL) Red 40 32 1.0 C 4.0 Yellow 5 40 3.0 20 Blue 1 8.0 V 1.0 8.0 Cf = µM V mL V = mL * TOOLS II
5. n° of 2-chloro-2-methylpropane is 1.3850. If a 1g sample of 2-chloro-2-methylpropane was contaminated with 1 drop of ethanol, what do you think the effect would be on the refractive index and by approximately how much? (1 drop of ethanol weighs about 0.03 g).
In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.
Which of the following terms describe how fast light travels through the medium? O All are correct Angular aperture O Limit of resolution Refractive index
1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.
2. Imagine you have mixed the following known concentrations of a solution. You then measured their absorbance in the spectrophotometer. Construct a standard curve (a line) from the following data points. Use the graph below. Each axis must be labeled with the variable and units, and the scale must be evenly spread. Tube Known concentration (M) Absorbance (AU) 1 0. 0.1 0.03 0.5 0.06 1.0 0.08 5 5.0 0.28 Eseel 22
Four lab groups completed the experiment listed in the lab diluting lul of FCF dye into 1ml of water. The A625 values are listed below. For brilliant blue FCF, ɛ =97000 M-1cm–1 MW = 792.8 g/mol %3D Group # A625 % solution (in original stock sample) Percent ror (True value of Stock=0.2%) 1 0.244 0.199 0.5 0.209 0.170 15 3 0.306 0.250 25 4 0.187 0.153 0.235 a. Which group was more accurate? b. What are possible reasons group 3 had a much higher Absorbance than the others? c. What are possible reasons group 4 had a much lower Absorbance than the others?
Dilute silver nitrate is sometimes applied to the eyes of newborn infants. Explain why a dilute solution is used rather than concentrated.
How many ml of a 40% solution can be prepared from 42.8 mg of sucrose? How many ml of a 40% solution can be prepared from 42.8 mg of sucrose? Question 3 options: b. 0.107 µl e. 1.09 µl a. 1.11 µl c. 10.08 µl d. 2.08 µl
A doctor orders 7 mg of compazine, which is used to treat nausea vertigo, and migraine headaches. If the stock solution is 1.5% (m/v) how many millilitres are administered to the patient? Express your answer to one significant figure and include the appropriate units... I want handwritten solution....i will up vote

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1. 540 nm	A. Wavescan
2. Quantity of light of a specific wavelength absorbed by a sample	B. Standard series
3. Reference samples with a concentration range	C. Absorbance
4. Absorbance values at all wavelengths	D. Purple

Match the following items: [4] 1. 540 nm A. Wavescan 2. Quantity of light of a specific wavelength absorbed by a sample B. Standard series 3. Reference samples with a concentration range C. Absorbance 4. Absorbance values at all wavelengths D. Purple