Make 500 ml of 10 mM Tris-HCl; pH=7.5; 0.25 M Glucose; 0.1% Tween-20; 5% BSA Molecular weight of Tris-HCl = 157.6 g/mol. You have a 3M glucose stock. Tween-20 is a liquid. BSA is a solid.   How many grams of Tris-HCl is used? How much of the 3M stock is used? How much of tween-20 is used? How much BSA is used? What is the process in making this solution?

Chemistry & Chemical Reactivity
9th Edition
ISBN:9781133949640
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter16: Principles Of Chemical Reactivity: The Chemistry Of Acids And Bases
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  1. Make 500 ml of 10 mM Tris-HCl; pH=7.5; 0.25 M Glucose; 0.1% Tween-20; 5% BSA

Molecular weight of Tris-HCl = 157.6 g/mol.

You have a 3M glucose stock.

Tween-20 is a liquid.

BSA is a solid.

 

How many grams of Tris-HCl is used?

How much of the 3M stock is used?

How much of tween-20 is used?

How much BSA is used?

What is the process in making this solution?

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