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Make 200 mL of 0.5 M HEPES pH 7.3 using 1.0 M HEPES-acid (pK, -7.6) and 1.0 M HEPES-base... Select the correct answer below: O Combine 73 mL of HEPES base with 127 mL of HEPES acid O Combine 34 mL of HEPES base with 66 mL of HEPES acid and 100 mL of water O Combine 10 mL of HEPES base with 40 mL of HEPES acid and 150 mL of water O Combine 63 mL of HEPES base with 27 mL of HEPES acid and 100 mL of water

Make 200 mL of 0.5 M HEPES pH 7.3 using 1.0 M HEPES-acid (pK, -7.6) and 1.0 M HEPES-base... Select the correct answer below: O Combine 73 mL of HEPES base with 127 mL of HEPES acid O Combine 34 mL of HEPES base with 66 mL of HEPES acid and 100 mL of water O Combine 10 mL of HEPES base with 40 mL of HEPES acid and 150 mL of water O Combine 63 mL of HEPES base with 27 mL of HEPES acid and 100 mL of water

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Make 200 mL of 0.5 M HEPES pH 7.3 using 1.0 M HEPES-acid (pKa =7.6) and 1.0 M HEPES-base. Select the correct answer below: O Combine 73 mL of HEPES base with 127 mL of HEPES acid Combine 34 mL of HEPES base with 66 mL of HEPES acid and 100 mL of water Combine 10 mL of HEPES base with 40 mL of HEPES acid and 150 mL of water O Combine 63 mL of HEPES base with 27 mL of HEPES acid and 100 mL of water
Transcribed Image Text:Make 200 mL of 0.5 M HEPES pH 7.3 using 1.0 M HEPES-acid (pKa =7.6) and 1.0 M HEPES-base. Select the correct answer below: O Combine 73 mL of HEPES base with 127 mL of HEPES acid Combine 34 mL of HEPES base with 66 mL of HEPES acid and 100 mL of water Combine 10 mL of HEPES base with 40 mL of HEPES acid and 150 mL of water O Combine 63 mL of HEPES base with 27 mL of HEPES acid and 100 mL of water
Expert Solution
Step 1: Determine the volumes of each species present in the solution:

Given,

Molarity of HEPES = 0.5 M

volume of the solution = 200 mL

pH of the solution = 7.3

Molarity of HEPES-acid = 1.0 M

Molarity of HEPES-base = 1.0 M

pKa of HEPES-acid = 7.6

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