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A: Density of the gas = 1.15 g/ml Volume of HF = 42 ml Mass of SiO2 = 54.06 g
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List 5 similarities and differences between oxidative phosphorylation and light reactions in the chloroplast
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- For the following questions, choose one to discuss: chloroplast/photosynthesis State at the outset which one you will discuss. A) What role do proton gradients play in the process of photosynthesis proton gradients allow B) Where are proton gradients formed? Within what structures are they seen in chloroplasts How do the structures help them to maintain a gradient? C) Explain where and how the chloroplast or mitochondria uses passive transport and active transport to complete photosynthesis or cellular respiration.Consider the structures and functions of mitochondria and chloroplasts. For each of the statements below, identify which part(s) of the chloroplast or mitochondrion (identified by letters in the figure below) are described. Some answers may include more than 1 letter. In those cases, separate the letters by a single space (eg. c g) Electron transport chains are located in ------ Photosystem I and II are located in ------- Ubiquinone is located in ------- NADPH is produced in ------ Pyruvate oxidation takes place in ------ ATP is produced in ------ NAD+ is produced in ----- High H+ concentration is produced in ----- O2 is produced in ----- RuBP is produced in ------(c) Compare the differences between oxidative phosphorylation and photophosphorylation by redrawing (if necessary) and completing the table given below: Table 1: Comparison of oxidative phosphorylation and photophosphorylation Oxidative phosphorylation Photophosphorylation Organelle Source of electrons Final electron acceptor Source of energy Role of ATP
- Eukaryotic chloroplasts generate exactly how many product molecules, in their consumption of six substrate carbon dioxide molecules and twelve water molecules, during oxygenic photosynthesis? twelve C6H12O6 molecules, six H2O molecules, and three CO2 molecules are produced one C6H12O6 molecule, six H2O molecules, and six O2 molecules are produced six C6H12O6 molecules, three H2O molecules, and six CO2 molecules are produced twelve H2O molecules and six O2 molecules are produced six H2O molecules and twelve CO2 molecules are producedDaniel Arnon and coworkers carried out experiments with intact, salt- washed chloroplasts to study photophosphorylation. When the chloroplasts were illuminated in the presence of ADP +P, ATP was produced, but oxygen was not produced or nor consumed. ATP formation was not accom- panied by a measurable electron transport involving any external electron donor or acceptor. The overall reaction for this result is: ADP + P -", ATP When NADP+ was included in addition to the ADP + P, ilumination of the intact chloroplats again resulted in resulted in the photophosphorylation of ADP to ATP. In addition, the NADPpt was reduced to NADPH + H* and o, was produced. Moreover, the light-induced reduction of NADP* was greatly decreased if ADP + P; were ommitted. The equation for this reaction is: NADP* + H,0 + ADP + R -", NADPH + H + ATP + }02 Briefly describe the mechanism(s) of these two types of photophosphoryla- tion that explain all of these results.Transport across the inner membrane of chloroplasts depends on the proton gradient established during ATP synthesis, similar to mitochondria. Can someone explain why this statement is false?
- Which of the following correctly sequences the steps of non-cyclic electron transport? * Water is oxidized by the capture of light energy; these excited electrons are passed through the dark reactions, returning to chlorophyll during the final light reactions The ATP and NADPH generated by the reactions of photosystem Il and photosystem I are utilized by the Calvin Cycle to build high energy glucose molecules Electrons donated from water molecules pass through photosystem I then O photosystem II before returning to the chlorophyll molecules, generating ATP in the process Chlorophyll molecules absorb UV radiation exciting electrons which flow through photosystem I, returning to the chlorophyll molecules ) This is a required questionIdentify the chemical basis for ApH and AY across the chloroplast thylakoid membrane by dragging the descriptions to their targets. Be sure to notice that the upper arrow iindicates ApH and the lower arrow indicates ΔΨ. ATP synthase complex H+ N ADP + P₁ Light energy ATP H*N Photosystem I/II- Chloroplast N side Aus PN ApH T + Thylakoid membrane HTp H+p Lumen Stroma P side Proton circuit A B High H concentration Low positive charge High positive charge Low H+ concentration Within the image, identify the types of proton translocation by dragging each label to its target. O XH₂ 2H+ + Z 2 H* ZH₂ O XH₂ Z 2H+ ZH₂ 2H+ C A B Proton pump Redox loopDescribe in detail the roles of different modes of membrane transport played in the photosynthesis in chloroplasts.
- Draw a sketch of a chloroplast and indicate where each of the following is located: CFoCF1, P700, P680, and Calvin cycle reactions. Describe the function of each.Plant chloroplasts are able to generate which of the following inorganic product molecules during oxygenic photosynthesis? cell biology C6H12O6 CO CO2 O2 H2S2.) A.) Explain how electron transfer leads to ATP biosynthesis in the light reactions of photosynthesis. You must give details about how electron flow builds a pH gradient and a description of the compartments in the chloroplast in relation to pH. Also, compare the ETC of the mitochondrion with the ETC of the light reactions for both the source and destination for electrons. B.) Would ATP biosynthesis happen if ionophores, which would create holes in the thylakoid membrane and allow uncontrolled passage of charged molecules such as protons between compartments, are provided to the chloroplast? Why or why not? C.) How would you modify Complex I of the electron transport chain to decrease ATP yield from Step 3 of β-oxidation? As part of this, you must explain why there is a difference in ATP yield between NADH and FADH 2 AND give a specific change to the ETC that would decrease ATP yield from Step 3