Light Cone (a) (b) Figure 26.17 (a) The numerical aperture (NA) of a microscope objective lens refers to the light-gathering ability of the lens and is calculated using half the angle of acceptance 0. (b) Here, a is half the acceptance angle for light rays from a specimen entering a camera lens, and D is the diameter of the aperture that controls the light entering the lens.
Light Cone (a) (b) Figure 26.17 (a) The numerical aperture (NA) of a microscope objective lens refers to the light-gathering ability of the lens and is calculated using half the angle of acceptance 0. (b) Here, a is half the acceptance angle for light rays from a specimen entering a camera lens, and D is the diameter of the aperture that controls the light entering the lens.
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You are using a standard microscope with a 0.10NA 4X objective and switch to a 0.65NA 40X objective. What are the acceptance angles for each? Compare and comment on the values. Which would you use first to
locate the target area on of your specimen? (See Figure 26.17.)
Transcribed Image Text:Light
Cone
(a)
(b)
Figure 26.17 (a) The numerical aperture (NA) of a microscope objective lens refers to the light-gathering ability of the lens and is
calculated using half the angle of acceptance 0. (b) Here, a is half the acceptance angle for light rays from a specimen entering a camera
lens, and D is the diameter of the aperture that controls the light entering the lens.
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