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- PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…Simulated data: Imagine you collect the following images at the above magnifications. Figure 3-2: Images of cheek cells at the above magnifications. Labelling Drawing: Label only the microscope image taken at 1000x magnification. Add lines and labels (as per guidelines for scientific drawings). You should include: A scale bar showing a unit length of 20 µm. (Hint: The length of the human cheek cell shown is 60 um). Labels for the cell membrane, nucleus, and cytoplasm.Immunfluorescence for Confocal Microscopy
- True or False: Ultrathin sections are used for light microscopy but are to thin to be stable under an electron mocroscope X-ray beam.Discuss about What is contrast harmonic Imaging?The basic component of a fluoroscopic x-ray system suitable for interventional procedures consist of a generator,x-ray tube and housing including collimator and filtration,patient table,anti-scatter grid and an image receptor.Describe the key design features and function of each of these component and briefly discuss the role of automatic brightness control
- Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron micrograph (SEM)The basic components of a fluoroscopic x-ray system suitable for interventional procedures consists of a generator,x-ray tube and housing including collimator and filtration,patient table,anti-scatter grid and an image receptor.Describe the key design features and function each of these components and briefly discuss the role of automatic brightness controlPlease decribe two image in complete sentences what models showns
- You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…Previous question: The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question. - Answer for this question was 3mm.Define stimulated emission depletion (STED) microscopy technique.