Lactose used up Growth on lactose Glucose used up Glucose and lactose added Growth on glucose Time of incubation (hr) FIGURE 7.24 Diauxic Growth Curve of E. coli Growing in a Medium Containing Glucose and Lactose Cells preferentially use glucose. Only when the supply of glucose is used up do cells start metabolizing lactose. Note that the growth on lactose is slower than it is on glucose. Number of cells (logarithmic scale)

Q: You have 2 organisms, A and B. One is an obligate aerobe and the other is an obligate anaerobe. You…

A: Microorganisms are categorized based on their oxygen requirement: Obligate aerobes Obligate…

Q: How does E coli grow in liquid medium?

A: The liquid medium is used to grow the bacteria culture in the test tube and also it will involve the…

Q: Biofilms often consist of multiple species of bacteria.True or false?

A: Bacteria are the prokaryotic organisms. They are unicellular type.

Q: List the four phases of the bacterial growth curve:

A: Microbes in a culture can be grown in order to obtain a large number of cells. Bacterial growth is…

Q: List the different type of bacteria depending on the salt concentration required for their growth?…

A: Halophiles are found in  three domains -Inside the Bacteria  halophiles depend on salt concentration…

Q: What effect does decreasing the incubation temperature have on bacterial growth rate? Why?

A: Bacterial growth is proliferation of bacterium into two daughter cells, in a process called binary…

Q: Your lab coordinator has decided to grow and maintain a sourdough starter. A sourdough starter is a…

A: In many species of reptile for example, no fixed temperature is necessary, but the actual for sake…

Q: In the exponential phase of microbial growth, Log cell numbers Time 1 1 1 1 1 1 I 1 1 a) net cell…

A: Microbial growth curveThe microbial growth curve shows the pattern of microbial growth.There are…

Q: In microbiology, the term growth in a culture usually refers to an increase in O 1) the number and…

A: Microbes are the microscopic appearance creatures that are not visible to the naked eye. These…

Q: Some exconjugants did not grow on minimal medium. On what medium would they grow?

A: Exconjugants can be described as bacterial cells, which separates following the conjugation. A…

Q: How do bacteria differ in terms of optimal temperature for growth?

A: The optimum temperature of the bacteria is defined as the suitable temperature where the maximum…

Q: Why are microorganisms called extremophiles?

A: Microorganisms are simplest form of organisms that cannot be seen by the unaided eye and are present…

Q: esence or absence of oxygen. After 12 hours, all of the glucose in each culture has been ned. You…

A: 1) The cell culture grown aerobically with the presence of oxygen will have more cell density than…

Q: What are the requirements to support microbial growth?

A: Microbial growth refers to growth (increases in the number of cells) of a microbial population. In…

Q: If a bacterial species is not susceptible to an antibacterial drug at the concentration present in a…

A: Antibiotic susceptibility test is the test used to determine the effect of antibiotic to an…

Q: You have a cell suspension in an isotonic medium. Explain in detail what would happen when you add:…

A: Urea mannitol acts at a hyperosmolar agent. Both mannitol and hypertonic saline are established as…

Q: What is a biofilm? What is the advantage for microbes to form a biofilm

A: A community of microorganisms which are attached to a surface irreversibly is called a biofilm. They…

Q: Why would hypertonic solution make a potato stick become limper?

A: Introduction: Osmosis is diffusion of water or solvent through a semi-permeable membrane from the…

Q: By accident, you placed a mesophile organism in a growth medium at pH 8. You expected it to die but…

A: Mesophiles are microorganisms that thrive at temperatures between 20 and 45 degrees Celsius, with an…

Q: Explain the purpose and phase where cells lose flagella and motility in biofilm formation, and why…

A: There are several steps associated with the biofilm formation. The first step includes adherence of…

Q: What factors can cause a lag phase in a bacterial growth curve?

A: The bacterial growth curve represents the number of live cells in a bacterial population over a…

Q: Why does a one-step growth curve differ in shapefrom that of a bacterial growth curve?

A: Growth curves are generally the description of the density of cell populations in liquid culture…

Q: An obligate halophile is an organism that requires high osmotic pressure.True or false?

A: Osmotic pressure refers to the minimum pressure that is required to stop the occurrence of osmosis…

Q: In some cases, why does a chemical is more effective as a microbicide  when it is diluted rather…

A: An antimicrobial is an agent that kills microorganisms or stops their growth. Antimicrobial…

Q: You inoculate 100 facultative anaerobic cells onto two media plates. You incubate one aerobically…

A: Introduction  Bacteria are the smallest microscopic unicellular organisms on the earth. Bacteria can…

Q: List 5 temperature requirements for bacterial growth. Explain the temperature ranges and where do…

A: Ans: Bacterial growth: The growth of animalcules or microbe happen at different optimum conditions.

Q: How would a facultative anaerobe growth curve change if it was placed in the presence of oxygen…

A: Facultative anaerobes are organisms that survive in the presence of oxygen and use oxygen for…

Q: Would it be advantageous for the microbe to be a a) heterotroph or b) autotroph? Explain your answer

A: All living organisms need food as a source of energy for carrying out various life processes.

Q: d) Since we know this bacterial organism thrives in colder areas of the world, what can be said…

A: Bacteria are present everywhere in the world, including cold environments. Certain types of bacteria…

Q: Name two biological processes that operate in a fixed film-growth mode?

A: This model considers secondary clarifier at a rural sewage treatment plant which is a typical phase…

Q: List and define the four phases of growth in a bacterial growth curve

A: the four phases of growth in a bacterial growth curve: There are four distinct phases of microbial…

Q: What cell types would be able to grow on the ECM in the following situations? F- , Hfr , Mix   a)…

A: Introduction  The ECM (extracellular matrix) is a complex mixture of proteins, glycoproteins, and…

Q: In what phase of the bacterial growth curve do cells die at rapid rates?

A: The bacterial growth is the complex process which involves anabolic and catabolic reactions which…

Q: Your lab coordinator has decided to grow and maintain a sourdough starter.  A sourdough starter is a…

A: A sourdough starter is "fed" flour and water every week to keep it alive. Each week, some of the…

Q: What are the effects of the different agar concentrations, the temperature, and the dye types on the…

A: Definition: internet passive motion of molecules from place of better awareness to place of decrease…

Q: Would all microorganisms exhibit the same bacterial growth curve? Why?

A: The bacterial growth curve is a closed curve, it is typical in nature for all microorganisms. All…

Q: If triclosan is the active agent in antibacterial handsoaps, what group of bacteria can grow freely…

A: Antibacterial soap is the one that contains the chemical ingredients which assist in killing the…

Q: if a bacterium lacks cytochrome C oxidase, does that make the bacterium an anaerobe? Explain?

A: Cytochrome c oxidase  is an enzyme present in the cytochrome sub unit. This is found in the electron…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.