Jessica's final year project deals with the metagenomic studies of anfH homologous genes from freshwater lake samples in Kampar Eastlake. She successfully cloned and sequenced two partial anfH homologous gene sequences. BlastX analyses revealed that one of the DNA sequences showed highest similarity to AnfH protein from a Brazilian soil bacteria isolate while the second sequence showed highest identity to the AnfH protein sequence of a bacterium that was isolated from Tonle Sap, a freshwater lake in Cambodia. To determine their evolutionary relationship, she subsequently constructed a phylogenetic tree using the corresponding protein sequences. Describe the steps that are required to perform the analysis.
Jessica's final year project deals with the metagenomic studies of anfH homologous genes from freshwater lake samples in Kampar Eastlake. She successfully cloned and sequenced two partial anfH homologous gene sequences. BlastX analyses revealed that one of the DNA sequences showed highest similarity to AnfH protein from a Brazilian soil bacteria isolate while the second sequence showed highest identity to the AnfH protein sequence of a bacterium that was isolated from Tonle Sap, a freshwater lake in Cambodia. To determine their evolutionary relationship, she subsequently constructed a phylogenetic tree using the corresponding protein sequences. Describe the steps that are required to perform the analysis.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Transcribed Image Text:Jessica's final year project deals with the metagenomic studies of anfH
homologous genes from freshwater lake samples in Kampar Eastlake. She
successfully cloned and sequenced two partial anfH homologous gene sequences.
BlastX analyses revealed that one of the DNA sequences showed highest
similarity to AnfH protein from a Brazilian soil bacteria isolate while the second
sequence showed highest identity to the AnfH protein sequence of a bacterium
that was isolated from Tonle Sap, a freshwater lake in Cambodia. To determine
their evolutionary relationship, she subsequently constructed a phylogenetic tree
using the corresponding protein sequences. Describe the steps that are required to
perform the analysis.
Expert Solution
Step 1
Phylogeny is the study of evolutionary relationships between different groups of organisms by tracing the evolutionary history of all the life forms present on the planet.
Phylogenetic tree is a diagram that helps to represent the evolutionary relationships among organisms. The branching pattern of this tree shows the evolutionary trends of the species evolved from the common ancestors.
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