is it essential that the primary stain and counterstain be of contrasting colors

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…

Q: What are the principle and basic concepts of NEGATIVE staining?

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: Why must young cultures be used when doing a Gram stain?

A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…

Q: How can the thickness of the smear affect the staining procedure?

A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…

Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…

A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…