In which parent and during which meiotic division did non-disjunction occur? If there is more than one correct answer, list all correct answers.  You may assume that non-disjunction occurred in only one parent.

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In which parent and during which meiotic division did non-disjunction occur? If there is more than one correct answer, list all correct answers.  You may assume that non-disjunction occurred in only one parent.

### Genetic Engineering and Plasmid Mapping

#### Restriction Site Mapping of a DNA Sequence

The first diagram illustrates the restriction enzyme mapping of a linear DNA segment. Restriction enzymes are proteins used to cut DNA at specific sequences. Here, different genes (Gene B, Gene I, and Gene O) are marked along with their corresponding restriction sites for various enzymes.

- **XhoI, SmaI, and PstI:** These restriction enzymes cut DNA within the **Gene B** region.
- **EcoRI and SalI:** These enzymes have cutting sites associated primarily with **Gene I**.
- **BamHI:** This enzyme cuts within the **Gene O** region.

#### pL311 Plasmid Map

The second diagram represents a circular plasmid, **pL311**. Plasmids are small, circular pieces of DNA that replicate independently of chromosomal DNA in bacteria. Key features of this plasmid include:

- **lacZ Gene (blue highlighted region):** Contains multiple restriction sites for enzymes such as BamHI, EcoRI, SmaI, and XhoI.
- **kan^r (Kanamycin resistance gene, blue highlighted region):** Essential for selecting transformed cells that have successfully taken up the plasmid.
- **ori (Origin of replication):** Necessary for the replication of the plasmid within the host cell.
- **PstI restriction site:** Located near the origin of replication.
- **HindIII and SalI:** Additional restriction sites associated with the kanamycin resistance gene.

By understanding these diagrams, students and researchers can identify where specific restriction enzymes will cut within the DNA sequence and how plasmid vectors are structured for genetic engineering applications. This knowledge is essential for cloning, gene editing, and various molecular biology experiments.
Transcribed Image Text:### Genetic Engineering and Plasmid Mapping #### Restriction Site Mapping of a DNA Sequence The first diagram illustrates the restriction enzyme mapping of a linear DNA segment. Restriction enzymes are proteins used to cut DNA at specific sequences. Here, different genes (Gene B, Gene I, and Gene O) are marked along with their corresponding restriction sites for various enzymes. - **XhoI, SmaI, and PstI:** These restriction enzymes cut DNA within the **Gene B** region. - **EcoRI and SalI:** These enzymes have cutting sites associated primarily with **Gene I**. - **BamHI:** This enzyme cuts within the **Gene O** region. #### pL311 Plasmid Map The second diagram represents a circular plasmid, **pL311**. Plasmids are small, circular pieces of DNA that replicate independently of chromosomal DNA in bacteria. Key features of this plasmid include: - **lacZ Gene (blue highlighted region):** Contains multiple restriction sites for enzymes such as BamHI, EcoRI, SmaI, and XhoI. - **kan^r (Kanamycin resistance gene, blue highlighted region):** Essential for selecting transformed cells that have successfully taken up the plasmid. - **ori (Origin of replication):** Necessary for the replication of the plasmid within the host cell. - **PstI restriction site:** Located near the origin of replication. - **HindIII and SalI:** Additional restriction sites associated with the kanamycin resistance gene. By understanding these diagrams, students and researchers can identify where specific restriction enzymes will cut within the DNA sequence and how plasmid vectors are structured for genetic engineering applications. This knowledge is essential for cloning, gene editing, and various molecular biology experiments.
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