In the Sanger (dideoxy) method for DNA sequencing, a small amount of a dideoxynucleotide triphosphate-say, ddCTP–is added to the sequencing reaction along with a larger amount of the corresponding dCTP along with the corresponding dATP, DTTP, dGTP nucleotides. What result would be observed if the dCTP were omitted? The strand produced will only contain A,T, and C residues When the first C residue is encountered in the template, ddCTP will be added, and polymerization will halt. When the first G residue is encountered in the template, ddCTP will be added, and polymerization will halt. If there are 7 G residues in the template there will be 7 fragments ending in a C residue. No reaction takes place.

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