In experiments carried out in 1941 to investigate the citric acid cycle, oxaloacetate labeled with ¹4C in the carboxyl carbon atom farthest from the keto group was introduced to an active preparation of mitochondria. COO CH₂ COO™ Oxaloacetate Analysis of the a-ketoglutarate formed showed that none of the radioactive label had been lost. Decarboxylation of x-ketoglutarate then yielded succinate devoid of radioactivity. All the label was in the released CO₂. Use an asterisk (*) to indicate the location of ¹4C in the intermediates and products of the pathway that converts [¹4C] oxaloacetate into succinate.
In experiments carried out in 1941 to investigate the citric acid cycle, oxaloacetate labeled with ¹4C in the carboxyl carbon atom farthest from the keto group was introduced to an active preparation of mitochondria. COO CH₂ COO™ Oxaloacetate Analysis of the a-ketoglutarate formed showed that none of the radioactive label had been lost. Decarboxylation of x-ketoglutarate then yielded succinate devoid of radioactivity. All the label was in the released CO₂. Use an asterisk (*) to indicate the location of ¹4C in the intermediates and products of the pathway that converts [¹4C] oxaloacetate into succinate.
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.7: The Metric System
Problem 4RQ
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