pls explain why
What will be the effect on resolution if the ionic strength of the buffer initially used
as mobile phase for elution in IEC was too high?
a. Increase
b. Decrease
c. No effect
d. indeterminate
In an SDS-PAGE experiment, protein X has an Rf = 0.75, while protein Y has an Rf
= 0.30. Which of the following statements are FALSE during the electrophoretic
run?
I. X has a greater charge-to-mass ratio than Y, since X has a smaller MW.
II. X has a lower MW, and thus experiences less friction with the gel matrix.
III. X has more hydrophobic groups, thus is in a more linearized state.
IV. X is globular, while protein Y is rod-like, thus X has higher mobility.
V. X is affected by less magnitude of electric field because of smaller MW.
a. I and V
b. II and IV
c. II, III, and IV
d. I, III, IV, and V
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