In an experiment, you grow bacterial cells for several generations in the presence of a light isotope of carbon (C¹2) and then transfer them into a medium containing a heavy isotope of carbon (C¹3). After one round of DNA replication, which of the following statements is true? O All DNA molecules will be labeled entirely with C¹3 For all DNA molecules, each strand will be labeled with a mixture of C¹2 and C¹3. O Half the DNA molecules will be labeled with C¹2 only, whereas the other half will be labeled with C¹3³ only. O All DNA molecules will be labeled entirely with C¹2 O For all DNA molecules, one strand will be entirely labeled with C¹2, whereas the other strand will be entirely labeled with C¹3 O Insufficient information to conclude.
In an experiment, you grow bacterial cells for several generations in the presence of a light isotope of carbon (C¹2) and then transfer them into a medium containing a heavy isotope of carbon (C¹3). After one round of DNA replication, which of the following statements is true? O All DNA molecules will be labeled entirely with C¹3 For all DNA molecules, each strand will be labeled with a mixture of C¹2 and C¹3. O Half the DNA molecules will be labeled with C¹2 only, whereas the other half will be labeled with C¹3³ only. O All DNA molecules will be labeled entirely with C¹2 O For all DNA molecules, one strand will be entirely labeled with C¹2, whereas the other strand will be entirely labeled with C¹3 O Insufficient information to conclude.
Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Transcribed Image Text:### DNA Replication Experiment Using Isotopes of Carbon
#### Experiment Description
In an experiment, you grow bacterial cells for several generations in the presence of a light isotope of carbon (C¹²) and then transfer them into a medium containing a heavy isotope of carbon (C¹³). After one round of DNA replication, consider the following statements. Which one is true?
#### Statements
1. All DNA molecules will be labeled entirely with C¹³.
2. **For all DNA molecules, each strand will be labeled with a mixture of C¹² and C¹³.**
3. Half the DNA molecules will be labeled with C¹² only, whereas the other half will be labeled with C¹³ only.
4. All DNA molecules will be labeled entirely with C¹².
5. For all DNA molecules, one strand will be entirely labeled with C¹², whereas the other strand will be entirely labeled with C¹³.
6. Insufficient information to conclude.
#### Explanation of the Correct Statement
The correct statement is: **For all DNA molecules, each strand will be labeled with a mixture of C¹² and C¹³.**
When bacterial cells initially grown in C¹² are transferred to a C¹³ medium and allowed to undergo one round of DNA replication, each resulting DNA molecule consists of one original strand (which contains C¹²) and one newly synthesized strand (which contains C¹³). This follows the principle of semi-conservative replication, where each new DNA molecule contains one strand from the original DNA and one new strand.
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