In a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the DNA structure. What is the fundamental physical basis for the absorbance increase? a. the effective concentration of DNA changes b. the absorbance of DNA is highly sensitive to temperature c. native DNA absorbs light more strongly than denatured DNA because the bases are stacked d. denatured DNA absorbs light more strongly than native DNA because the bases have become unstacked

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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In a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the DNA structure. What is the fundamental physical basis for the absorbance increase?

a. the effective concentration of DNA changes

b. the absorbance of DNA is highly sensitive to temperature

c. native DNA absorbs light more strongly than denatured DNA because the bases are stacked

d. denatured DNA absorbs light more strongly than native DNA because the bases have become unstacked

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