(iii) Based on the double-stranded DNA sequence of terminator, draw the structure of hairpin loop that will be formed during transcription. (iv) Illustrate how the hairpin loop structure initiates the termination of transcription.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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iii and iv

Shown below is a very short gene of an unknown bacteria genome (Figure 2). Transcription
starts at Transcription Start Site (TSS) and terminates at the Terminator site.
TSS
5 TATTATAACGCATGAGGAGCCATGCATTATCGGTATATGCACTGACCCGGAAAGGCTCCTTTTGGAGCCTTTTTT-3′
3' ATAATATTGCGTACTGCTCGGTACGTAATAGCCATATACGTGACTGGGCCTTTTCCGAGGAAAACCTCGGAAAAA-5
Promoter
Terminator
Figure 2
(i)
Which strand of DNA (the top or the bottom) is used by RNA polymerase as a template?
What are the amino acids translated from the resulting mRNA? Indicate the amino
(NH3) and carboxyl (COO) termini of the protein.
(iii)
Based on the double-stranded DNA sequence of terminator, draw the structure of
hairpin loop that will be formed during transcription.
(iv)
Illustrate how the hairpin loop structure initiates the termination of transcription.
Transcribed Image Text:Shown below is a very short gene of an unknown bacteria genome (Figure 2). Transcription starts at Transcription Start Site (TSS) and terminates at the Terminator site. TSS 5 TATTATAACGCATGAGGAGCCATGCATTATCGGTATATGCACTGACCCGGAAAGGCTCCTTTTGGAGCCTTTTTT-3′ 3' ATAATATTGCGTACTGCTCGGTACGTAATAGCCATATACGTGACTGGGCCTTTTCCGAGGAAAACCTCGGAAAAA-5 Promoter Terminator Figure 2 (i) Which strand of DNA (the top or the bottom) is used by RNA polymerase as a template? What are the amino acids translated from the resulting mRNA? Indicate the amino (NH3) and carboxyl (COO) termini of the protein. (iii) Based on the double-stranded DNA sequence of terminator, draw the structure of hairpin loop that will be formed during transcription. (iv) Illustrate how the hairpin loop structure initiates the termination of transcription.
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