(ii) Consider the following reaction: Acetoacetate + NADH + H* + B-hydroxybutyrate + NAD* The reduction of acetoacetate by NADH is a coupled redox reaction. The half- reactions are: Acetoacetate + 2 H" + 2e + B-hydroxybutyrate E"= - 0.346V NAD* + H* + 2e + NADH E"=- 0.315V Calculate AE and AG for this reaction at 25°C when [acetoacetate] and [NADH] are 10 mM and 9 mM respectively, and [B-hydroxybutyrate] and [NAD'] are 1 mM and 1.ImM respectively. (R= 8.315 J/K/mol, F= 96.485 kJ/V/mol)
(ii) Consider the following reaction: Acetoacetate + NADH + H* + B-hydroxybutyrate + NAD* The reduction of acetoacetate by NADH is a coupled redox reaction. The half- reactions are: Acetoacetate + 2 H" + 2e + B-hydroxybutyrate E"= - 0.346V NAD* + H* + 2e + NADH E"=- 0.315V Calculate AE and AG for this reaction at 25°C when [acetoacetate] and [NADH] are 10 mM and 9 mM respectively, and [B-hydroxybutyrate] and [NAD'] are 1 mM and 1.ImM respectively. (R= 8.315 J/K/mol, F= 96.485 kJ/V/mol)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
answer ii
![If 0.1 M glucose 1-phosphate is incubated with phosphoglucomutase, the glucose 1-
phosphate is converted to glucose 6-phosphate. At equilibrium, the concentration of
glucose l-phosphate is 4.5 x 103 M and that of glucose 6-phosphate is 8.6 x 102 M.
Calculate Keq' and AG for this reaction (assume it is in the direction of glucose 6-
phosphate formation). T= 298 K, R= 8.315 J/K/mol.
Glucose 1- phosphate + Glucose 6-phosphate
(ii)
Consider the following reaction:
Acetoacetate + NADH + H* + B-hydroxybutyrate + NAD*
The reduction of acetoacetate by NADH is a coupled redox reaction.
The half- reactions are:
Acetoacetate + 2 H" + 2e + B-hydroxybutyrate
E"= - 0.346V
NAD* + H* + 2e + NADH
Eo=- 0.315V
Calculate AE and AG for this reaction at 25°C when [acetoacetate] and [NADH] are
10 mM and 9 mM respectively, and [B-hydroxybutyrate] and [NAD*] are 1 mM and
1.ImM respectively.
(R= 8.315 J/K/mol, F= 96.485 kJ/V/mol)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F875cac72-0f82-4647-92a7-f5dce3b969ce%2F569ae507-a7a2-4c17-b042-440fe244d7c9%2F1u9fxyk_processed.jpeg&w=3840&q=75)
Transcribed Image Text:If 0.1 M glucose 1-phosphate is incubated with phosphoglucomutase, the glucose 1-
phosphate is converted to glucose 6-phosphate. At equilibrium, the concentration of
glucose l-phosphate is 4.5 x 103 M and that of glucose 6-phosphate is 8.6 x 102 M.
Calculate Keq' and AG for this reaction (assume it is in the direction of glucose 6-
phosphate formation). T= 298 K, R= 8.315 J/K/mol.
Glucose 1- phosphate + Glucose 6-phosphate
(ii)
Consider the following reaction:
Acetoacetate + NADH + H* + B-hydroxybutyrate + NAD*
The reduction of acetoacetate by NADH is a coupled redox reaction.
The half- reactions are:
Acetoacetate + 2 H" + 2e + B-hydroxybutyrate
E"= - 0.346V
NAD* + H* + 2e + NADH
Eo=- 0.315V
Calculate AE and AG for this reaction at 25°C when [acetoacetate] and [NADH] are
10 mM and 9 mM respectively, and [B-hydroxybutyrate] and [NAD*] are 1 mM and
1.ImM respectively.
(R= 8.315 J/K/mol, F= 96.485 kJ/V/mol)
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