If you were using the quadrant streak plate method to plate a very dilute broth culture (with many fewer bacteria than the broth used for the plate pictured here), would you expect to see single, isolated colonies in quadrant 4 or quadrant 3? Explain your answer.

Q: The number of bacteria in a sample can also be quantified with other techniques, such as examination…

A: In microbiology laboratories the number of microorganisms present in a particular sample is…

Q: A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had…

A: The number of generations (n) refers to the number of times a cell population got doubled during a…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: In a bacterial culture on an agar plate, what is a zone of inhibition?     An area where the…

A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.

A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: Collecting data that can then be transformed into a bacterial growth curve is usually done using a…

A: A spectrophotometer is an instrument used to measure the amount of light absorbed by a sample. The…

Q: Why is a metal loop heat to read before picking up a bacterial sample? What should you do with a…

A: In microbiology, inoculation is the technique of transferring bacteria into culture media so that…

Q: Can you tell IF the pasteurization process is working in this milk producing plant? How can you…

A: Pasteurization is a process in which milk or milk product is heated to a certain temperature for a…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: Using a dilution series is a way to transfer the very small volumes of original culture necessary to…

A: Answer -To determine the volume of the original bacterial culture required to obtain a specific…

Q: 1) both streak plating and pour plating produce isoluated colonies. What is the underlying…

A: Hello there! Since you have posted multiple questions and not mentioned which one of them to be…

Q: Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown…

A: Endospores are dormant, tough, vegetative structure produced by the bacteria from the Firmicute…

Q: You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this…

A: Bacteria are prokaryotic organism and divides by binary fission that is responsible for doubling the…

Q: The process of collecting characteristics of your unknown bacterium can be helped by the use of a…

A: Bacteria refer to single-celled microscopic organisms. They are found to be powerful and complex…

Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…

A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: Based on these results, which of the following statements is true regarding the effectiveness of…

A: A disinfectant is a chemical substance or compound that is used to inactivate or destroy the…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: How is the correct way of incubating culture plate media? Explain why is that so.

A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…

Q: You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal…

A: The evaluation of fecal microscopic organisms in water could be a critical public well-being measure…

Q: You perform several tests on an unidentified bacterial species and get the results below. Based on…

A: Bacterial identification is the process of determining the specific type, species, or strain of…

Q: You have a mixed culture containing 4 different organisms that you ihocl plate of media. When you…

A: Media or culture medium is a liquid or gel that is specifically designed to support the growth of…

Q: If you counted 300 colonies on a plate that had a 10-5 dilution of bacteria culture, how many…

A: Introduction  Colonies are groups of microorganisms, such as bacteria or yeast, growing on or within…

Q: You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method…

A: Microbiological research necessitates the use of pure cultures. Spreading bacteria on the surface of…

Q: Why should bacterial culture plates be incubated inverted (bottom up)?

A: Microbial isolation is a process done in microbiology lab in order to obtained microbe of interest…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.