If you needed to make 250 mL of a 1x TBE buffer (final concentrations of 89 mM Tris, 89 mM boric acid, 2.0 mM EDTA), how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50 M EDTA?
If you needed to make 250 mL of a 1x TBE buffer (final concentrations of 89 mM Tris, 89 mM boric acid, 2.0 mM EDTA), how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50 M EDTA?
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter15: Acid–base Equilibria
Section: Chapter Questions
Problem 95AP
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If you needed to make 250 mL of a 1x TBE buffer (final concentrations of 89 mM Tris, 89 mM boric acid,
2.0 mM EDTA), how much of each of the following would you use: solid Tris base (121.1 g/mol); solid
boric acid (61.8 g/mol); liquid stock solution of 0.50 M EDTA?
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Hi, for the No. of Moles = given mass/molar mass why would you multiply the given mass by 1000?
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