If you needed to make 250 mL of a 1x TBE buffer (final concentrations of 89 mM Tris, 89 mM boric acid, 2.0 mM EDTA), how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50 M EDTA?

Chemistry: The Molecular Science
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ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter15: Additional Aqueous Equilibria
Section: Chapter Questions
Problem 93QRT: When 40.00 mL of a weak monoprotic acid solution is titrated with 0.100-M NaOH, the equivalence...
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 If you needed to make 250 mL of a 1x TBE buffer (final concentrations of 89 mM Tris, 89 mM boric acid,
2.0 mM EDTA), how much of each of the following would you use: solid Tris base (121.1 g/mol); solid
boric acid (61.8 g/mol); liquid stock solution of 0.50 M EDTA?

 

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Hi, for the No. of Moles = given mass/molar mass why would you multiply the given mass by 1000?

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