If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
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- You are given two proteins with a pI of 4.5 and pI of 7.7. In an example of an anion-exchange column, can you use a buffer to separate these two proteins? What buffer protein can be used to separate the two proteins mentioned above?What is the pH of a 0.25 M solution of acetic acid, Ka = 1.8 x 105. What percentage of the acid is dissociated?for 0.9% NaCl solution and 10% NaCl solution, which one has higher concentration of WATER?
- You have given two proteins with a pI of 4.5 and pI of 7.7? Using an anion-exchange column, how can these two proteins be separated. What buffer could you prepare?I want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?Refer to the following titration curve below: 13 12 11 10 7 5 4 3 2 8 10 12 14 16 18 20 22 24 26 28 30 Volume of Titrant / mL Unknown Acid 0.10 mol/L - titrant = NaOH 0.1 mol/L How many buffering regions are present in the titration curve of the amino acid?
- When 2.43 g of a nonelectrolyte solute is dissolved in water to make 435 mL of solution at 25 °C, the solution exerts an osmotic pressure of 895 torr. What is the molar concentration of the solution? concentration: 0.045 M Incorrect How many moles of solute are in the solution? moles of solute: 0.034 mol Incorrect What is the molar mass of the solute? molar mass: 69.12 g/mol IncorrectWhat is the pH of a buffer that contains 0.9 M ammonia and 0.3 M ammonium ion? (Ka for ammonium ion = 5.8 x 10-10Three buffers are made by combining a 1M solution of acetic acid and a 1M solution of sodium acetate in the ratios shown in the table below. Which of these statements is true regarding the prepared buffers? (Ka= 1.7x10-5) pH of buffer 1 < pH of buffer 2 < pH of buffer 3 pH of buffer 1 = pH of buffer 2 = pH of buffer 3 pH of buffer 1 > pH of buffer 2 > pH of buffer 3 pH of buffer 1 = pH of buffer 2 > pH of buffer 3 pH of buffer 1 > pH of buffer 2 = pH of buffer 3
- The pl of alkaline phosphatase is 4.5; the pl of the DEAE cellulose is 10.5. We used a buffer of pH 7.4 to run a DEAE-cellulose as anion exchange column? Explain how did this work?A mixture of three proteins was subjected to ion exchange chromatography using CM-cellulsoe as the stationary phase. The elution buffer had a pH of 7.5. Proteins were eluted by increasing the ionic strength of the buffer. Predict the order of elution of the following proteins:Pepsinogen, pI = 1Cytochrome c, pI = 10.6Myoglobin, pI = 6.8What is the pH of a 0.150 M solution of acetic acid (pK₂ = 4.75) at 25 °C?